2D-NMR研究中国生漆漆酚结构

2D-NMR Studies on the Structure of Urushiol of Chinese Lacquer

用离心旋转薄层层析法在涂渍AgNO3的硅胶板上成功地分离了乙酰化漆酚。对其主要组分(64.6%),用^(1)H、^(13)C常规谱,DEPT编辑谱,同核、异核化学位移相关二维谱,二维J谱,异核中继相干传递二维谱及弛豫时间T_(1)等,确定了此化合物的结构与构型和^(1)H、^(13)C谱线归属。实验证明漆酚中此组分是3-十五碳烯烃(8′Z、11′E、13′Z)邻苯二酚,异核中继相干传递二维谱新技术能有效地应用于复杂未知化合物的结构测定。

The urushiol diacetatcs have been successfully separated on the silica gel coated with silver nitrate by centrifugal rotation TLC.The structure and configuration of key component of urushiol(64.4%)were determined successfully by 1H and 13C conventional methods,DEPTtechnique,homo and beleronuclcar chemical shift correlation 2D-NMR,2D-JNMR,helcronuclear relayed coherence transfer 2D-NMR,and relaxation time T_(1) investigation.The assignment of resonances for ^(1)H and ^(13)C were confirmed.Plenty of cvidence shows that the key component of urushiol is 3-(8'Z,11'E,13'Z-pentadccatrienyl)catechol.The results show that RELAY 2D-NMR new technique can be successfully applied to determining the structure of complicated unknown compounds.