单纯形优化高效液相色谱法分离测定维生素E的各种异构体

Simplex Optimization of HPLC to Separate and Determine Vitamin E Isomers

本文用计算机辅助单纯形优化高效液相色谱法分离、测定了精炼植物油副产物中维生索E的各种异构体。探讨了单纯形优化法指导高效液相色谱选择分离的实验条件,并编写了实用的计算机程序。实验结果表明,本法可使比较困难的条件选择工作有章可循,从而提高工作效率。

The paper describes HPLCmethod for the determination ofα-,β-,γ-,δ-isomers of Vitamin Ein by-products of refined plant oil.Asimplex method wasadopted to optimize the chromatographic conditions and a practical program was worked out.The experimental results are acceptable and the simplex method is proved to be very useful for selection of conditions in HPLC.