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Expanding the scope of genome editing with SpG and SpRY variants in rice 被引量：7

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摘要 Dear Editor,The clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9)system,since it was excavated,has been rapidly developed and sparked a revolution in the genome editing field.In principle,CRISPR/Cas9 system relies on the recognition of specific loci on the genome,which is titled the protospacer adjacent motif(PAM).However,the canonical Streptococcus pyogenes Cas9(SpCas9)nuclease only recognizes NGG or NAG PAMs,rendering an inherent obstacle in amplifying the application of CRISPR/Cas9 technology.
作者 Jun Ren Xiangbing Meng Fengyue Hu Qing Liu Yuexuan Cao Huiying Li Changjie Yan Jiayang Li Kejian Wang Hong Yu Chun Wang
机构地区 State Key Laboratory of Rice Biology State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) University of Chinese Academy of Sciences Key Laboratory of Plant Functional Genomics
出处 《Science China(Life Sciences)》 SCIE CAS CSCD 2021年第10期1784-1787,共4页 中国科学（生命科学英文版）
基金 supported by the National Transgenic Science and Technology Program(2019ZX08010-003) the Central Publicinterest Scientific Institution Basal Research Fund(Y2020XK17) the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
关键词 Cas9 NUCLEASE RENDERING
分类号 S511 [农业科学—作物学] Q943.2 [生物学—植物学]
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共引文献87

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引证文献7

二级引证文献24

Science China(Life Sciences)
2021年 第10期

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