基于生物信息学分析宫颈癌中TGFBI的表达及临床意义

The Expression and Clinical Significance of TGFBI in Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma Based on Bioinformatics Analysis

为探究转化生长因子-β诱导基因(TGFBI)在宫颈癌(CESC)中的表达及临床意义,利用Oncomine、GEPIA、LinkedOmics、Kaplan-Meier Plotter数据库及Ualcan数据库分析TGFBI在CESC中mRNA及蛋白的表达情况,TGFBI表达与CESC临床特征的关系及其对CESC患者预后的影响,TGFBI在肿瘤组织中的甲基化情况。利用Timer数据库分析TGFBI基因与肿瘤免疫细胞浸润的关系。结果显示:TGFBI在CESC中高表达;在CESC组织中TGFBI甲基化表达水平较正常宫颈组织降低;TGFBI表达与CESC患者的各临床病理参数之间具有一定的相关性,TGFBI表达与CESC患者的组织类型、种族、年龄、肿瘤纯度、放疗、T分期、N分期、M分期、淋巴结转移数量相关(P<0.05)。生存分析表明,TGFBI高表达使CESC患者的总体生存及无病生存率降低(P<0.05)。本研究阐明了TGFBI在CESC组织中高表达可作为CESC诊断、治疗效果及评估预后的判定指标之一。

To explore the expression of transforming growth factor-βinduced gene(TGFBI)in cervical squamous cell carcinoma and endocervical adenocarcinoma(CESC)and its relationship with clinical significance,Oncomine,GEPIA,LinkedOmics,Kaplan-Meier Plotter and Ualcan databases were used to analyze the expression of TGFBI in CESC,the relationship between TGFBI expression and clinical characteristics of CESC,its impact on the prognosis of patients with CESC,and the methylation of TGFBI in tumor tissue.Timer was used to analyze the relationship between TGFBI gene and tumor immune cells infiltration.The results have indicated that the expression level of TGFBI in CESC tissues was higher than that in normal cervical tissues;the methylation level of TGFBI in CESC tissue was lower than that in normal cervical tissues.There is a significant correlation between the expression of TGFBI and the clinicopathological parameters of CESC patients.The expression of TGFBI was singnificantly correlated with histological type,race,age,tumor purity,radiotherapy,T stage,N stage,M stage and the number of lymph node metastasis(P<0.05).The overall survival and progression-free survival were significantly shorter in the TGFBI overexpression group than that in the low expression group(P<0.05).The study was conducted to elucidate that TGFBI is highly expressed in CESC and is associated with the occurrence and development of CESC,which greatly increases the possibility of TGFBI as a potential therapeutic target.