稀土钕掺杂介孔生物玻璃的微球调控实验

Experiment on Tailoring the Properties of Neodymium-doping Mesoporous Bioactive Glass Nanospheres

采用溶胶-凝胶法制备稀土钕(Nd)掺杂介孔生物玻璃(Nd/MBG),用扫描电镜、透射电镜等仪器分析不同Nd掺杂比对Nd/MBG微球药物控释能力及生物安全性的影响。结果表明,Nd/MBG为分布均匀的微球,表面存在纳米孔隙。改变Nd掺杂量可调控Nd/MBG微球的大小及孔径。Nd/MBG微球浸泡模拟体液后能够体外矿化,具有良好的生物活性。Nd/MBG微球能够装载阿霉素(DOX),并在不同pH中表现出差异化的控释能力。体外细胞培养结果显示Nd/MBG微球对骨肉瘤细胞(MG63)的生物安全性具有剂量依赖性,当浓度低于100μg/mL时,表现出较高的安全性。Nd/MBG-DOX微球能够控制DOX的释放,抑制MG63的生长。通过改变Nd掺杂比调控制备Nd/MBG微球的药物控释能力和生物安全性,在药物载体领域具有潜在应用价值。

In this study,a novel ion neodymium doped mesoporous bioactive glasses nanospheres(Nd/MBG)was successfully synthesized by a facile sol-gel technique.The drug release capacity and biosafety of Nd/MBG microspheres were examined by scanning electron microscopy(SEM),transmission electron microscopy(TEM)and so on.The results show that Nd/MBG microsphere has uniform spherical morphology with nano-pore on the surface.The size and structure of Nd/MBG microsphere are adjusted by the amount of Nd-doped.The Nd/MBG microspheres can be mineralized hydroxyapatite after immersion in simulated body fluids(SBF),showing good biological activity.Nd/MBG microspheres depends on the dose of Nd3+,and shows the controllable doxorubicin(DOX)release ability in different pH values.In vitro cell cultures show that Nd/MBG nanospheres have dose dependent cytotoxicity to MG63 osteosarcoma cells.The Nd/MBG microsphere shows higher biosafety when the concentration is lower than 100 g/mL.Meanwhile,the release of DOX from the Nd/MBG-DOX is controlled,which inhibits the growth of MG63 cells.These all note that the synthesized Nd/MBG nanospheres have good controlled-release ability and biological safety after regulating the ratio of Nd-doped,which can be used as a filling biomaterial for the drug carrier.