DRR1与TMOD2在人宫颈癌细胞HeLa中相互作用的研究

Interaction between DRR1 and TMOD2 in human cervical cancer cell line HeLa

目的本研究旨在阐明肾细胞癌下调蛋白1(DRR1)与原肌球调节蛋白2(TMOD2)在人宫颈癌HeLa细胞中的相互作用。方法利用DNA重组技术构建不同长度的DRR1蛋白片段的表达载体,并将各载体分别转染HeLa细胞,采用免疫印迹技术对不同长度的DRR1蛋白表达片段进行验证。将不同长度DRR1片段的表达载体分别与TMOD2-HA表达载体共转染到HeLa细胞中,利用免疫共沉降方法检测不同长度的DRR1蛋白片段与TMOD2蛋白相互结合的情况。结果在HeLa细胞中,DRR1(1-90)蛋白片段,DRR1(1-120)蛋白片段和全长DRR1(1-144)蛋白可以与TMOD2蛋白结合。结论DRR1蛋白质的不同部位与TMOD2蛋白具有不同的结合能力。

Objective To investigate the interaction between down-regulated in renal cell carcinoma 1(DRR1)and tropomodulin-2(TMOD2)in HeLa cells.Methods We constructed different DRR1 fragmentexpressing vectors,and transfected them into HeLa cells respectively.The expression of different DRR1 fragments was determined by western blot.Then,the different DRR1 fragment-expressing vectors and TMOD2-HA expressing vector were transfected into HeLa cells,and co-immunoprecipitation was performed to test the interaction of the different DRR1 fragments and TMOD2.Results DRR1(1-90)fragment,DRR1(1-120)fragment and full length DRR1(1-144)protein can bind to TMOD2 in HeLa cells.Conclusion Different domains of DRR1 show different binding ability with TMOD2.