全自动酶标仪检测冠心病患者血清CysC、LP(a)、hs-CRP的应用及其相关性

Application and Correlation of Automatic Microplate Reader in the Detection of Serum CysC,LP(a)and hs-CRP in Patients with Coronary Heart Disease

目的:探讨全自动酶标仪对冠心病不同病情程度患者血清胱抑素C(CysC)、脂蛋白a[LP(a)]及高敏C反应蛋白(hs-CRP)的检查结果及其相关性。方法:选择2020年1月~2020年10月本院收治的100例冠心病患者为观察组,另选取30例健康体检志愿者为对照组,采用Bio-RAD 550型全自动酶标仪对患者CysC、LP(a)、hs-CRP进行检测,并比较不同病情程度冠心病患者血清CysC、LP(a)、hs-CRP浓度及其与冠心病病情程度的相关性。结果:观察组血清CysC、LP(a)、hs-CRP高于对照组(P<0.05);病情重度患者血清CysC、LP(a)、hs-CRP高于中度及轻度患者(P<0.05);病情中度患者血清CysC、LP(a)、hs-CRP高于轻度患者(P<0.05);经Pearson分析显示,血清CysC、LP(a)、hs-CRP与冠心病病情程度均呈正相关性(P均<0.05)。结论:应用Bio-RAD 550型全自动酶标仪的检测结果发现,冠心病不同病情程度患者血清CysC、LP(a)、hs-CRP表达存在明显差异,CysC、LP(a)、hs-CRP浓度与病情程度均呈正相关性。

Objective:To investigate the results and correlation of serum cystatin C(CysC),lipoprotein a[Lp(a)]and high sensitivity C-reactive protein(hs-CRP)in patients with coronary heart disease(CHD).Methods:100 cases of patients with CHD in our hospital from January 2020 to October 2020 were selected as the observation group,and 30 healthy volunteers were selected as the control group.The levels of CysC,LP(a)and hs CRP in patients with coronary heart disease(CHD)were detected by Bio-RAD 550 automatic enzyme labeling instrument,and the correlation between serum CysC,LP(a),hs-CRP and severity of coronary heart disease was compared.Results:Serum CysC,LP(a)and hs-CRP in the observation group were higher than those in the control group(P<0.05);serum CysC,LP(a)and hs-CRP in severely ill patients were higher than those in moderate and mild patients(P<0.05);serum CysC,LP(a),hs-CRP in patients with moderate disease is higher than that in mild patients(P<0.05);pearson analysis showed that serum CysC,LP(a),hs-CRP and the severity of coronary heart disease were all positively correlated(all P<0.05).Conclusion:The results of Bio-RAD 550 automatic enzyme labeling instrument showed that,the expression of serum CysC,LP(a)and hs-CRP in patients with coronary heart disease is significantly different.The concentration of CysC,LP(a)and hs-CRP is positively correlated with the severity of coronary heart disease.