叶酸中蝶酸含量控制工艺研究

Study on controlling butterfly acid content in folic acid

以N-(4-氨基苯甲酰)-L-谷氨酸、2,5,6-三氨基-4-羟基嘧啶硫酸盐、三氯丙酮为原料,合成叶酸粗品,叶酸粗品经酸精制及碱精制得叶酸成品。因叶酸酸溶时可以水解成蝶酸,所以研究了酸精制过程中稀硫酸用量、稀硫酸浓度、及稀硫酸精制时间对成品中蝶酸含量的影响。实验结果表明,酸精制过程中稀硫酸用量为叶酸粗品的10倍、稀硫酸浓度为30%、及在稀硫酸溶液中精制时间为1 h,叶酸成品中蝶酸含量明显降低,目标化合物经液相色谱分析,纯度可达99.5%,蝶酸含量可以降至0.18%。

Folic acid was synthesized from N-(4-aminobenzoyl)-l-glutamic acid,2,5,6-triamino-4-hydroxypyrimidine sulfate and trichloroacetone.The effects of the amount of dilute sulfuric acid,the concentration of dilute sulfuric acid and the refining time of dilute sulfuric acid on the content of butterfly acid were studied.The results showed that e concentration of dilute sulfuric acid was 10 times of crude folic acid,the concentration of dilute sulfuric acid was 30%,and the time of refining in dilute sulfuric acid solution was 1 hour,the purity of the target compound was 99.5%,and the content of papillary acid was reduced to 0.18%.