泽兰提取物调控NDRG2对低糖低氧所致的神经细胞损伤的影响

Effect of extract of Lycopus lucidus Turcz protect the cell from neuronal ischemic injury model by targeting NDRG2

[目的]研究泽兰提取物对低糖低氧所致神经细胞损伤的影响及潜在分子机制。[方法]用PC12细胞建立脑缺血模型,然后用0.1、1、10 mg/mL的泽兰提取物对模型细胞进行处理,噻唑蓝(MTT)法测定PC12细胞的光密度(OD)值,蛋白免疫印迹法(Western blot)检测N-Myc下游调控基因2(NDRG2)、细胞周期蛋白1(Cyclin D1)、p21、Bax和Bcl-2蛋白表达,流式细胞术测定细胞凋亡率。[结果]与对照组相比,1 mg/mL和10 mg/mL泽兰提取物均可使模型组PC12细胞OD值升高,CyclinD1和Bcl-2表达升高,NDRG2、p21和Bax表达降低,促细胞增殖并抑制细胞凋亡;抑制NDRG2表达可促进模型组PC12细胞增殖并抑制细胞凋亡;过表达NDRG2可逆转泽兰提取物对模型组PC12细胞增殖和凋亡的作用。[结论]泽兰提取物通过抑制NDRG2表达促进神经细胞脑缺血损伤模型细胞PC12增殖,抑制细胞凋亡。泽兰提取物可能对神经细胞脑缺血损伤具有潜在的治疗作用。

[Objective]To study the effect and potential molecular mechanism of Lycopus lucidus Turcz extract on nerve cell damage caused by low glucose and hypoxia.[Methods]PC12 cells were used to establish cerebral ischemia injury model.Then the model cells were treated with Lycopus lucidus Turcz extract of 0.1,1 and 10 mg/mL.OD value of PC12 cells was measured by MTT assay.The expression levels of NDRG2,Cyclin D1,p21,Bax and Bcl-2 protein was detected by Western blot,and apoptotic rate was determined by flow cytometry.[Results]Compared with the control group,both 1 mg/mL and 10 mg/mL Lycopus lucidus Turcz extract increased OD value of PC12 cells in the model group,increased expression of CyclinD1 and Bcl-2,decreased expression of NDRG2,p21 and Bax,promoted cell proliferation and inhibited apoptosis.Inhibition of NDRG2 promoted proliferation and inhibited apoptosis of PC12 cells in the model group.Overexpression of NDRG2 reversed the effects of Lycopus lucidus Turcz extract on proliferation and apoptosis of PC12 cells in the model group.[Conclusion]Lycopus lucidus Turcz extract promoted the proliferation and inhibited the apoptosis of cerebral ischemia injury model cells PC12 by inhibiting NDRG2.Lycopus lucidus Turcz extract may have potential therapeutic effects on cerebral ischemic injury of nerve cells.