低能量脉冲电磁场对金黄色葡萄球菌生长的影响及其与抗生素的 协同抑菌作用

Effects of low-energy pulsed electromagnetic field on the growth of Staphylococcus aureus and its synergistic antibacterial effect with antibiotics

目的:探讨低能量脉冲电磁场(PEMF)对体外金黄色葡萄球菌(ATCC 29213)生长的影响及其与抗生素是否有协同作用。方法:实验分两部分。(1)分析不同作用时长的PEMF对金黄色葡萄球菌生长的影响。将一定量金黄色葡萄球菌接种至普通营养肉汤,分为对照组和PEMF干预组(场强1 Gs,频率15 Hz,脉宽5μs,脉冲群宽5 ms,脉冲群不应期62 ms),两组均置于37℃温箱培养,每隔2 h取样倍比稀释后接种至哥伦比亚血平板,37℃温箱培养,24 h后计数。(2)分析PEMF与抗生素对金黄色葡萄球菌的联合影响。分为对照组、PEMF组、抗生素组、PEMF+抗生素组;4组均置于37℃温箱培养,8 h后取样倍比稀释并接种至哥伦比亚血平板,37℃温箱培养,24 h后计数。结果:(1)2 h、4 h的PEMF干预与对照组比较,菌落计数差异无统计学意义(P>0.05);6 h的PEMF干预后,菌落计数为对照组的23%(P<0.01);8 h的PEMF干预后,菌落计数为对照组的28%(P=0.01)。(2)PEMF组、抗生素组、PEMF+抗生素组菌落计数分别是对照组的32%、34%,10%,都显著少于对照组;同时,PEMF+抗生素组菌落计数显著少于PEMF组和抗生素组。结论:低能量PEMF可显著抑制对数生长期金黄色葡萄球菌,同时与抗生素之间具有协同抑菌作用。

Objective To analyze the effect of low-energy pulsed electromagnetic field(PEMF)on the growth of Staphylococcus aureus(ATCC 29213)in vitro,and to investigate whether low-energy PEMF has synergistic antibacterial effect with antibiotics.Methods The experiment was divided into two parts.(1)Analyzing the effect of PEMF with different action durations on the growth of Staphylococcus aureus.Acertain amount of Staphylococcus aureus was inoculated into ordinary nutrition broth,and then divided into control group and PEMF intervention group in which Staphylococcus aureus was intervened with PEMF with a field strength of 1 Gs,frequency of 15 Hz,pulse width of 5μs,pulse group width of 5 ms and pulse group refractory period of 62 ms.Both two groups were cultured in 37℃incubator.The samples were collected every 2 hours,and after dilution,they were inoculated into Columbia blood plate in 37℃incubator and counted after 24 hours.(2)Analyzing the synergistic antibacterial effect of PEMF and antibiotics on Staphylococcus aureus.The Staphylococcus aureus was divided into 4 groups,namely control group,PEMF group,antibiotics group,and PEMF+antibiotics group.The 4 groups were cultured in a 37°C incubator.After 8 hours,the samples were diluted and inoculated into Columbia blood plates in 37°C incubator and counted after 24 hours.Results(1)There was no significant difference in colony count between PEMF interventions for 2 and 4 hours and control group(P>0.05).After PEMF intervention for 6 hours,the colony count in PEMF group was 23%of control group(P<0.01),and after PEMF intervention for 8 hours,the colony count in PEMF group was 28%of control group(P<0.01).(2)The colony count of PEMF group,antibiotics group and PEMF+antibiotics group were 32%,34%and 10%of control group,respectively,which were significantly less than that of control group.Meanwhile,the colony count of PEMF+antibiotics group was significantly less than that of PEMF group and antibiotics group.Conclusion The low-energy PEMF can significantly inhibit Staphylococcus aureus in the logarithmic growth period,and has a synergistic antibacterial effect with antibiotics.