摘要
目的探讨依达拉奉对间歇低氧大鼠海马CA1区神经细胞凋亡及凋亡相关蛋白半胱氨酸蛋白酶激动剂(Smac)与X染色体连锁的凋亡抑制蛋白(XIAP)表达的影响。方法随机将成年Wistar雄性大鼠96只分为对照组、5%间歇低氧组、依达拉奉组,每组分别分为7 d、14 d、21 d、28 d四个时间亚组,每个亚组8只。采用活性氧检测试剂盒对实验大鼠海马组织的活性氧含量进行检测;采用免疫组织化学法及蛋白免疫印迹法检测海马CA1区Smac、XIAP蛋白的表达;采用原位末端标记法对海马CA1区神经细胞的凋亡情况进行检测。结果与对照组比较,5%间歇低氧组和依达拉奉组各个时间点亚组海马区神经细胞活性氧含量、Smac、XIAP蛋白、神经细胞凋亡指数增加(均P<0.05),依达拉奉组活性氧含量、Smac蛋白的表达以及神经细胞凋亡指数明显低于5%间歇低氧组(均P<0.05),依达拉奉组XIAP蛋白的表达明显高于间歇低氧组(P<0.05)。结论依达拉奉可能通过清除氧自由基提高机体抗氧化能力、调控Smac及XIAP介导的细胞凋亡,进而发挥神经细胞保护作用。
Objective To explore effects of edaravone on apoptosis and expressions of apoptotic proteins Smac and XIAP in hippocampal CA1 pyramidal cell of rats under intermittent hypoxia.Methods A total of 96 adult male Wistar rats were randomly divided into control group,5%intermittent hypoxic group and edaravone group,and each group was divided into 4 time groups at 7 d,14 d,21 d and 28 d,respectively,with 8 rats in each subgroup.The content of reactive oxygen species(ROS)in hippocampal tissues of the experimental rats was detected by the reactive oxygen species detection kit.Immunohistochemistry and Western blot were used to detect the expressions of Smac and XIAP protein in hippocampal CA1 region.The Tunel method detected the apoptosis of neurons.Results Compared with the control group,the content of ROS,the expressions of Smac and XIAP proteins and the neuronal apoptosis index in the hippocampus were increased in the 5%intermittent hypoxia group and the edaravone group at each time point(all P<0.05).The content of ROS,the Smac protein expression and the neuronal apoptosis index in the edaravone group were significantly lower than those in the 5%intermittent hypoxia group(all P<0.05).The expression of XIAP protein in the edaravone group was significantly higher than that in the 5%intermittent hypoxia group(P<0.05).Conclusion Edaravone may improve the antioxidant capacity of the body by scavenging oxygen free radicals and regulate Smac and XIAP-mediated apoptosis,thus playing a protective role on neurons.
作者
王玲
王袁
张嘉宾
汪彦辉
张敏
WANG Ling;WANG Yuan;ZHANG Jiabin;WANG Yanhui;ZHANG Min(Department of Respiratory Medicine,North China University of Science and Technology Affiliated Hospital,Tangshan,Hebei 063000,P.R.China;Clinical Medical College,North China University of Science and Technology,Tangshan,Hebei 063000,P.R.China;Department of Respiratory Medicine,First Hospital of Qinhuangdao,Qinhuangdao,Hebei 066000,P.R.China)
出处
《中国呼吸与危重监护杂志》
CAS
CSCD
北大核心
2021年第9期632-636,共5页
Chinese Journal of Respiratory and Critical Care Medicine
基金
河北省医学科学研究课题(20201244、20210742)。