基于文献挖掘和网络药理学策略对中药肝毒性靶标机制的研究

The study of hepatotoxicity target of traditional Chinese medicine based on literature mining and network pharmacology strategy

运用文献挖掘与网络药理学策略筛选中药肝毒性靶标,为中药肝毒性机制研究与临床安全使用提供理论依据。对中国知网(CNKI)和PubMed中的中药肝毒性文献进行分析,并在此基础上采用网络药理学的方法筛选肝毒性核心靶标并进行毒性机制分析。共检索到相关文献3 864篇,对其中符合条件的60篇文献进行分析;网络药理学筛选到degree值大于4的靶标有谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)、碱性磷酸酶(ALP)、谷胱甘肽(GSH)、活性氧(ROS)、超氧化物歧化酶(SOD)、肿瘤坏死因子(TNF-α)、谷胱甘肽过氧化物酶(GSH-Px)。结果表明,ALT、AST、MDA、ALP、GSH、ROS、SOD、TNF-α、GSH-Px等可作为肝毒性机制研究的重要检测靶标,中药肝毒性机制与氧化应激密切相关。

The literature mining and network pharmacology were used to screen the liver toxicity targets of traditional Chinese medicine to provide evidence for clinical safety. CNKI and PubMed were used to retrieve massage that reported the hepatotoxicity of traditional Chinese medicine and then screened out the core of evaluation indicators of hepatotoxicity by network pharmacology. 3 864 references were retrieved and 60 references were analysed. Glutamic oxalacetic transaminase(ALT),glutamicpyruvic transaminase(AST),malonaldehyde(MDA),alkaline phosphatase(ALP),glutathione(GSH),reactive oxygen species(ROS),super oxidedismutase(SOD),tumor necrosis factor(TNF-α),glutathione peroxidase(GSH-Px)were screened because of the degree over 4 in network. ALT,AST,MDA,ALP,GSH,ROS,SOD,TNF-α and GSH-Px could provide the targets as the hepatotoxicity of traditional Chinese medicine. The mechanism that traditional Chinese medicine caused liver toxic is lactated to oxidative stress closely.