山东某高校新生亚健康状态与血浆免疫球蛋白G N-糖基组的相关性

Association between plasma IgG N-glycan profiling and suboptimal health status among undergraduates in a college in Shandong Province

目的探索山东某高校新生亚健康状态(suboptimal health status,SHS)与血浆免疫球蛋白G(IgG)N-糖基组之间的相关性。方法采用病例对照研究设计,根据纳入、排除标准选取2017年9月至11月于山东潍坊学院进行健康体检的大学生100例为研究对象,根据SHS评判标准,将研究对象分为SHS组(50例)与健康对照组(50例)。采用超高效液相色谱技术进行血浆IgG N-糖基组检测,获得24个糖基峰。IgG N-糖基组与SHS的关联分析采用Mann-Whitney U秩和检验及二分类Logistics回归分析;采用受试者工作特征曲线分析评估血浆IgG N-糖基作为SHS生物标志物的可能性。结果由24个糖基峰构成的IgG N-糖基组中的GP17与SHS差异存在统计学意义(P<0.05),且SHS人群GP17水平较高。基础模型的诊断效能(AUC)为0.826(95%CI:0.747~0.905,P<0.001);糖基模型的AUC为0.631(95%CI:0.519~0.744,P=0.002),基础模型纳入糖基变量后构建了联合模型,其AUC为0.848(95%CI:0.763~0.912,P<0.001),其诊断效能与基础模型相比呈现出提高的趋势。结论山东某高校新生SHS与IgG N-糖基组中的GP17水平存在关联,SHS人群GP17水平较高。

Objective To explore the association between suboptimal health status(SHS)and plasma IgG N-glycans levels among undergraduates in a college in Shandong Province.Methods A case-control study was conducted from September to November 2017,100 college students who underwent physical examinations at Weifang University in Shandong,were selected as study participants based on the inclasion and exclusion criteria of the study.According to the criteria of SHS,the participants were divided into an SHS group(n=50)and a health control group(n=50).Plasma IgG N-glycosylaton levels were analyzed by means of ultra-high liquid chromatography(UPLC),and 24 glycan peaks were obtained.The Mann-Whitney U-test and binary logistic regression analysis were performed to investigate the association between IgG N-glycans and SHS.P<0.05 was considered statistically significant.Receiver operating characteristic(ROC)curve analyses were used to evaluate the possibility of plasma IgG N-glycans being a biomarker of SHS.Results The results of univariate and multivariate analysis showed that GP17 was associated with SHS(P<0.05),and the relative abundance of initial glycan peaks(GP17)was higher in the SHS group compared with the control group.ROC curve analysis showed that the area under the curve(AUC)of the baseline model was 0.826(95%confidence interval[CI]:0.747-0.905,P<0.001);the AUC of the glycan-based model was 0.631(95%CI:0.519-0.744,P=0.002),and the AUC of the combined model was 0.848(95%CI:0.763-0.912,P<0.001).Compared with the baseline model,the diagnostic efficiency of the combined model revealed a trend of improvement.Conclusions The SHS of the students in a college in Shandong Province was associated with an IgG N-glycan level of GP17,which was significantly higher than that of the control group.