铜纳米颗粒的仿生合成及其在CT成像中的应用

The synthesis of biomimetic copper nanoparticles and its application in CT imaging

提出了一种简单易行的合成策略,以蛋清白蛋白(CEW)为稳定剂和生物模板来制备铜(Cu)纳米颗粒Cu@CEW,并就其在电子计算机断层扫描(CT)成像应用方面进行了初步探索.在温和实验条件下,通过一锅法合成的均匀分散的Cu@CEW纳米颗粒平均粒径为4.62 nm.通过稳定性测试表明:制备的Cu@CEW纳米颗粒具有良好的多溶液体系分散性和稳定性,放置7 d后无聚沉现象产生.通过生物毒性试验表明:Cu@CEW纳米颗粒具有良好的生物相容性,在人肺癌细胞A549与质量浓度为30μg·mL^(-1)的Cu@CEW纳米颗粒共孵育48 h后,仍保持着较高的细胞存活率.与临床造影剂碘海醇相比,Cu@CEW纳米颗粒具有比较明显的CT增强成像效果,这为金属Cu纳米材料的合成和应用提供了新的思路.

In this study,a simple and feasible method was proposed to prepare copper(Cu)nanoparticles(Cu@CEW)using egg albumin(CEW)as stabilizer and biological template,and it made a preliminary exploration on the application of computed tomography(CT).Under mild experimental conditions,the average particle size of uniformly dispersed Cu@CEW synthesized by one-pot method is 4.62 nm.The stability test show that the prepared Cu@CEW have better dispersion and stability in multisolution system,and there are no aggregation and sedimentation after 7 d of storage.At the same time,the biotoxicity test show that Cu@CEW have excellent biocompatibility,and under the experimental condition that human lung cancer cells A549 are coincubated with 30μg·mL^(-1)Cu@CEW for 48 h,A549 cells maintain a high survival rate.Compared with the clinical contrast agent iohexol,Cu@CEW have obvious CT-enhanced imaging effect.This provides a new idea for the synthesis and application of metallic copper nanomaterials.