基于一步法制备的纳米复合材料发展光电化学方法检测钙卫蛋白

Development of photoelectrochemical methods for the detection of calprotectin based on nanocomposites prepared by one-step method

金纳米粒子(Au NPs)敏化二氧化锡(SnO_(2))半导体,不仅拓宽了材料对可见光的吸收范围,且异质界面易形成肖特基势垒,有效促进光生电子空穴对e-/h+的分离,提高传感器的灵敏度.探索了一步合成法制备SnO_(2)@Au NPs异质材料,该材料具有较高的光电转化效率,表现出较好的光电性能.基于此纳米复合材料均匀牢固的特点,可在其表面修饰识别层,构建“三明治”夹心结构生物传感器,实现对钙卫蛋白(CP)的特异性光电测定.线性范围为0.01~20.00μg·mL^(-1),检测限低至3.2 ng·mL^(-1),且特异性好、稳定性佳.此外,传感器也成功用于检测实际血样中的CP,并获得了较好的回收率,可实现对CP的快速无创性检测.

Gold nanoparticles(Au NPs)-sensitized tin dioxide(SnO_(2))semiconductor not only broadens the visible light absorption range of the material,but also easily forms a Schottky barrier at the heterogeneous interface,which effectively promotes the separation of photogenerated e-/h+and improves the sensitivity of the sensor.This work explored the one-step synthesis method to prepare SnO_(2)@Au NPs heterogeneous materials,which have high photoelectric conversion efficiency and show better photoelectric properties.Based on the uniformity and firmness of this nanocomposite,the recognition layer can be modified on its surface to construct a“sandwich”biosensor with a sandwich structure to realize the specific photoelectric determination of calprotectin(CP).The linear range is 0.01-20.00μg·mL^(-1),the detection limit is as low as 3.2 ng·mL^(-1),and the specificity is good and stable.In addition,the sensor has also been successfully used to detect CP in actual blood samples and obtained a good recovery rate,which could realize rapid and non-invasive detection of CP.