LncRNA HCG22通过miR-629-5p/FOXO3轴抑制宫颈癌细胞增殖、迁移和侵袭

LncRNA HCG22 inhibits the proliferation, migration and invasion of cervical cancer cells through miR-629-5p/FOXO3 axis

目的本研究旨在探讨lnc RNA HCG22在宫颈癌的增殖、迁移和侵袭中的作用和机制。方法采用实时RT-q PCR检测宫颈癌组织和细胞系中HCG22和mi R-629-5p的表达。将He La细胞分为pc DNA3.1组、pcDNA3.1-HCG22组和pcDNA3.1-HCG22+miR-629-5p mimics组。通过MTT法、细胞划痕实验和Transwell小室实验评估He La细胞生长、迁移和侵袭。荧光素酶报告基因分析HCG22/mi R-629-5p和mi R-629-5p/FOXO3之间的相互作用关系。结果与癌旁组织或END1/E6E7细胞相比,HCG22在宫颈癌组织和细胞中均表达下调(P <0.01)。与pc DNA3.1组相比,上调HCG22表达显著抑制了宫颈癌细胞增殖(P <0.01)、迁移(P <0.01)和侵袭(P <0.01)。与癌旁组织或END1/E6E7细胞相比,mi R-629-5p在宫颈癌组织和细胞中表达上调(P <0.01),且宫颈癌组织中mi R-629-5p表达与HCG22呈负相关(r=-0.7661, P <0.01)。在pc DNA3.1-HCG22基础上转染mi R-629-5p mimics显著逆转了pc DNA3.1-HCG22对宫颈癌细胞增殖(P <0.01)、迁移(P <0.01)和侵袭(P <0.01)的抑制作用。HCG22和FOXO3均能够与mi R-629-5p靶向结合,pc DNA3.1-HCG22显著抑制了mi R-629-5p的表达(P <0.01),同时促进了FOXO3的m RNA表达(P <0.01)。结论 HCG22在宫颈癌中表达下调,而mi R-629-5p表达上调。HCG22能够通过mi R-629-5p/FOXO3轴,在宫颈癌的增殖、迁移和侵袭过程中发挥抑癌作用。

Objective To investigate the role and mechanism of lncRNA HCG22 in proliferation, migration and invasion of cervical cancer. Methods The expressions of HCG22 and miR-629-5 p in cervical tissues and cells were detected by RT-qPCR. HeLa cell were allocated into pcDNA3.1 group, pcDNA3.1-HCG22 group, and pcDNA3.1-HCG22 + miR-629-5 p mimics group. The HeLa cell growth, migration and invasion were assessed by MTT, woundhealing and Transwell assays, respectively. The interaction relationship between HCG22/miR-629-5 p and miR-629-5 p/FOXO3 were analyzed by luciferase reporter. Results HCG22 was significantly decreased in cervical cancer tissues and cell lines compared with paired paracancerous tissues or END1/E6 E7 cell(P < 0.01). The upregulation of HCG22 significantly inhibited tumor cell proliferation(P < 0.01), migration(P < 0.01), and invasion(P < 0.01) compared with pcDNA3.1 group. miR-629-5 p expression was increased in cervical cancer tissues and cell lines compared with paired paracancerous tissues or END1/E6 E7 cell(P < 0.01). There was a negative correlation between HCG22 and miR-629-5 p(r =-0.7661, P < 0.01). The addition of miR-629-5 p mimics reversed the suppressive effects of pcDNA3.1-HCG22 on cell proliferation, migration, and invasion(P < 0.01). Both HCG22 and FOXO3 bound to miR-629-5 p. pcDNA3.1-HCG22 inhibited miR-629-5 p(P < 0.01), while promoted mRNA expression of FOXO3(P < 0.01). Conclusions HCG22 was downregulated in cervical cancer, while miR-629-5 p was upregulated. HCG22 gave function as a tumor suppressor in proliferation, migration and invasion of cervical cancer by targeting miR-629-5 p/FOXO3 axis.