摘要
本论文研究了水酶法提取油茶壳中茶皂素的工艺参数,通过单因素试验考察酶添加量、料液比、提取时间、温度等因素对水酶法提取茶皂素的影响规律;然后,筛选三个显著性影响因素酶添加量、温度、浸提时间,根据中心组合试验(Box-Behnken)设计原理,以茶皂素提取率为响应值,优化茶皂素最佳提取工艺参数料液比1︰20,酶添加量2mg,温度60℃,浸提时间90min,在此条件下得到茶皂素的提取率为1.40%。
This thesis investigated the process parameters of aqueous enzymatic extraction of tea saponin from oil tea husk,and investigated the effect of enzyme addition,feed-to-liquid ratio,extraction time,temperature and other factors on aqueous enzymatic extraction of tea saponin by single-factor test;then,screened three significant influencing factors enzyme addition,temperature,and extraction time,according to the central combination test(Box-Behnken)design principle,with tea saponin extraction rate as The response value was used to optimize the optimal extraction process parameters of tea saponin;the material-to-liquid ratio of 1︰20,enzyme addition of 2 mg,temperature of 60℃,extraction time of 90 min,and maximum yield of 1.40%.
作者
袁成华
杨琼玲
邓爱华
李菊元
何婉琳
Yuan Chenghua;Yang Qiongling;Deng Aihua;Li Juyuan;He Wanlin(Hu’nan Kangduoli Oil Co.,Ltd.,Changde 415700;College of Life and Environment Science,Hu’nan University of Arts and Science,Changde 415000,China)
出处
《广东化工》
CAS
2021年第17期19-21,24,共4页
Guangdong Chemical Industry
基金
湖南省知识产权战略实施专项(油茶精深加工及综合利用知识产权密集型产业培育,20201016312F)
常德市重点研发计划项目(富硒油茶产业升级关键技术研究与应用,常财企指[2020]75号)。
关键词
油茶
茶皂素
水酶法
单因素实验
响应面设计
camellia oleifera
tea saponin
hydro-enzymatic method
single factor
responsive surface design