油茶果壳中茶皂素提取工艺研究

Study on Extraction Technology of Tea Saponin from Camellia Oleifera Shell

本论文研究了水酶法提取油茶壳中茶皂素的工艺参数,通过单因素试验考察酶添加量、料液比、提取时间、温度等因素对水酶法提取茶皂素的影响规律;然后,筛选三个显著性影响因素酶添加量、温度、浸提时间,根据中心组合试验(Box-Behnken)设计原理,以茶皂素提取率为响应值,优化茶皂素最佳提取工艺参数料液比1︰20,酶添加量2mg,温度60℃,浸提时间90min,在此条件下得到茶皂素的提取率为1.40%。

This thesis investigated the process parameters of aqueous enzymatic extraction of tea saponin from oil tea husk,and investigated the effect of enzyme addition,feed-to-liquid ratio,extraction time,temperature and other factors on aqueous enzymatic extraction of tea saponin by single-factor test;then,screened three significant influencing factors enzyme addition,temperature,and extraction time,according to the central combination test(Box-Behnken)design principle,with tea saponin extraction rate as The response value was used to optimize the optimal extraction process parameters of tea saponin;the material-to-liquid ratio of 1︰20,enzyme addition of 2 mg,temperature of 60℃,extraction time of 90 min,and maximum yield of 1.40%.