超高效液相色谱法测定淫羊藿中8种黄酮类成分的含量

Determination of 8 Flavonoids in Epimedium by UPLC

建立同时测定淫羊藿中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、淫羊藿新苷A、宝藿苷I、朝藿苷甲、朝藿苷丙等8种黄酮成分含量的超高效液相色谱测定方法。采用ZORBAX Eclipse XDB C_(18)柱(2.1 mm×50 mm,1.8μm)分离,以乙腈-0.1%甲酸为流动相梯度洗脱,流速为0.2 mL/min,进样量1μL,检测波长为270 nm,柱温30℃。8种黄酮成分可以实现完全分离,在0.2~20.0 mg/L范围内呈良好的线性关系,线性系数大于0.9991,样品加标平均回收率为94.6%~106.5%,相对标准偏差不大于5.0%。本方法快速,灵敏,准确,可用于淫羊藿中8种黄酮类成分的同时测定。

To establish a UPLC method for determination of 8 flavonoids(Epimedin A,Epimedin B,Epimedin C,Icariin,Epimedoside A,Baohuoside I,Epimedoside,Epimedin I)in Epimedium.The separation was performed on a ZORBAX Eclipse XDB C_(18) column(2.1 mm×50 mm,1.8μm),acetonitrile and 0.1% formic acid were used as mobile phase for gradient elution,the flow rate was 0.2 mL/min,injection volume was 1.0μL.Detection wavelength was set at 270 nm,the column temperature was set at 30℃.Eight flavonoids can be completely separated.Linear relationship of 8 flavonoids were good in the range of 0.2~20.0 mg/L.The linear coefficient is greater than 0.9991.The average recoveries were 94.6%~106.5%,the relative standard deviation is not greater than 5.0%.This method is rapid,sensitive,accurate,and it can be used for the determination of 8 flavonoids in Epimedium.