紫草素对宫颈癌细胞株Caski侵袭迁移及Wnt/β-catenin信号通路的影响

Effects of Shikonin on the Invasion and Migration of Cervical Cancer Cell Line Caski and Wnt/β -catenin Signaling Pathway

目的:探讨紫草素对宫颈癌细胞株Caski细胞侵袭迁移及Wnt/β-catenin信号通路的影响。方法:采用MTT法检测细胞增殖抑制情况;采用Transwell小室侵袭迁移实验检测Caski细胞侵袭迁移情况;采用qPCR方法检测Caski细胞GSK-3β、Wnt2B表达情况;采用Western bloting方法检测Caski细胞β-Catenin、p-β-Catenin蛋白含量。结果:紫草素可抑制Caski细胞的增殖,具有时间-浓度依赖性,并可抑制细胞的侵袭迁移,增加Caski细胞GSK-3βmRNA和p-β-Catenin蛋白的表达量,减少Caski细胞Wnt mRNA和β-Catenin蛋白的表达量。结论:紫草素可能通过抑制Caski细胞Wnt/β-Catenin信号通路抑制Caski细胞的侵袭迁移。

Objective:To explore the effects of shikonin on the invasion and migration of cervical cancer cell line Caski and Wnt/β-catenin signaling pathway.Methods:MTT method was used to detect the inhibition of cell proliferation;Transwell chamber invasion and migration experiment was adopted to measure the invasion and migration of Caski cells;qPCR method to measure the expressions of GSK-3βand Wnt2B in Caski cells;Western bloting method to measure the contents ofβ-Catenin and pβ-Catenin protein in Caski cells.Results:Shikonin could inhibit the proliferation of Caski cells,in a time-concentration dependent manner,it could inhibit cellular invasion and migration,in crease the expressions of GSK-3βmRNA and pβ-Catenin protein,and lower the contents of Wnt mRNA andβ-Catenin protein in Caski cells.Conclusion:Shikonin could restrain the invasion and migration of Caski cell possibly through inhibiting Wnt/β-catenin signaling pathway.