寄生离子对离子导入巩膜交联术核黄素渗透性及交联效果的影响

The effect of parasitic ions on riboflavin permeability and cross-linking effectiveness in iontophoresis-assisted scleral cross-linking

离子导入法中寄生离子对紫外交联效果的影响尚不清楚。本文采用核黄素乳酸钠林格液(A组和核黄素生理盐水(B组)作为离子导入溶液,对离体猪眼巩膜进行紫外交联,计算了溶液中寄生离子浓度;检测了核黄素导入后的平均荧光强度、渗透深度、渗透浓度以及交联后巩膜组织力学性能;推算了两种溶液的扩散系数范围。结果表明,A组溶液含寄生离子种类较B组多;渗透10 min后,B组核黄素平均荧光强度、渗透深度、渗透浓度及巩膜弹性模量均明显高于A组;B组核黄素的扩散系数约是A组的1.5倍。本文研究结果提示,寄生离子种类对核黄素导入的渗透性影响较大,并且还会进一步影响交联后巩膜组织的力学性能。基于以上研究结果,期望本研究可为今后施行离子导入巩膜交联术时提高核黄素导入效率、优化核黄素配方提供参考。

The effect of parasitic ions on the results of ultraviolet A(UVA) cross-linking in iontophoresis was still not clear. In this work, the porcine sclera was cross-linked by riboflavin lactate Ringer’s solution(group A) and riboflavin normal saline(group B) in vitro, respectively. The concentration of parasitic ions in the solution was calculated.In addition, the average fluorescence intensity, penetration depth and concentration after the introduction of riboflavin and the mechanical properties of cross-linked sclera tissue were measured. The ranges of diffusion coefficient of the two solutions were also calculated, respectively. The results showed that more kinds of parasitic ions were detected in group A compared with group B, while the average fluorescence intensity, penetration depth and concentration of riboflavin and scleral elastic modulus in group B were significantly higher than those in group A when the penetration time was 10 minutes. Besides, the diffusion coefficient of riboflavin in group B was about 1.5 times larger than that in group A. The results suggested that the species of parasitic ions has a great impact on the permeability of riboflavin, and affects the mechanical properties of cross-linked sclera. The above results could provide a reference for improving the efficiency of riboflavin introduction and optimizing the formula of riboflavin in iontophoresis scleral cross-linking.