光辐照稳定的光热-化疗联合的可注射紫杉醇凝胶

A photothermal/chemotherapy injectable paclitaxel gel with irradiation stability

本研究的目的是构建一种光辐照稳定的光疗与化疗相结合的可注射凝胶。将光敏剂IR780和抗氧化剂白藜芦醇(Res)引入紫杉醇(PTX)的聚乙二醇(PEG)溶液,制备了具有光疗与化疗性质的Res-PTX@IR780凝胶。结果表明,PTX、PTX@IR780和Res-PTX@IR780能够形成凝胶,其凝胶具有可注射性。ATR-FTIR结果既表明了凝胶的组成,又表明了凝胶的形成涉及氢键的作用。紫外光谱表明,IR780溶液不稳定,而Res-IR780溶液的光辐照稳定性明显提高。光热测试表明,Res-PTX@IR780比PTX@IR780凝胶有更好的光热转换效果以及多次辐照光热稳定性。小鼠体内探索结果显示,注射了Res-PTX@IR780凝胶的部位光辐照后从35℃升温达到64℃,温差达30℃。由于良好的光热转换和光热循环效果,Res-PTX@IR780凝胶作为光热治疗与化疗结合的材料用于肿瘤的原位治疗十分具有潜力。

The aim of this study is to construct an injectable gel with stable phototherapy and chemotherapy. ResPTX@IR780 gel with phototherapy and chemotherapy property was prepared by introduction of photosensitizer IR780 and antioxidant resveratrol(Res) into the polyethylene glycol(PEG) solution of paclitaxel(PTX). The results showed that PTX, PTX@IR780 and Res-PTX@IR780 could form gels and the gels were injectable. ATR-FTIR results indicated not only components of the gels but also the formation of hydrogen bonding during the gelation. The results of UV showed instability of IR780 solution and stability improvement of Res-IR780 solution under infrared radiation(IR) irradiation.Photothermal tests showed that Res-PTX@IR780 displayed better photothermal conversion and photothermal stability under multiple irradiations than PTX@IR780. The results of in vivo exploration in mice showed that the skin site injected with Res-PTX@IR780 gel heated up from 35 ℃ to 64 ℃, and the temperature difference was up to 30 ℃. Res-PTX@IR780 gel is very promising as a combination agent of photothermal therapy and chemotherapy for the in situ treatment of tumors due to good photothermal conversion and photothermal stability under multiple irradiations.