通过式高效净化-高效液相色谱-串联质谱法测定猪肉中噁唑菌酮残留

Determination of Famoxadone Residues in Pork by HPLC-MS/MS Coupled with Direct Pass Type Solid Phase Extraction

建立了猪肉中噁唑菌酮的通过式高效净化-高效液相色谱-串联质谱(HPLC-MS/MS)检测方法。样品经过1%甲酸乙腈提取,氮吹浓缩后加水复溶混匀,经Oasis PRIME HLB柱净化后,HPLC-MS/MS检测,基质标准曲线定量。噁唑菌酮在1~50μg·L-1浓度范围内线性良好,R^(2)>0.995,在0.01 mg·kg^(-1)、0.02 mg·kg^(-1)和0.05 mg·kg^(-1)3个添加水平下,噁唑菌酮的回收率为82%~89%,相对标准偏差为0.3%~1.1%。此方法具有简单、快速、灵敏、精密度好等特点,适用于动物源产品中噁唑菌酮残留的测定。

A method for the determination of famoxadone in pork by using HPLC-MS/MS coupled with direct pass type solid phase extraction was established.The sample was extracted with 1%acidified acetonitrile,concentrated,mixed with water,purified by oasis prime HLB column,detected by HPLC-MS/MS,and quantified by matrix standard curve.The famoxadone linearity was good in the concentration range of 1~50μg·L-1,R^(2)>0.995.At the addition levels of 0.01 mg·kg^(-1),0.02 mg·kg^(-1)and 0.05 mg·kg^(-1),the recovery of oxazolidone was 82%~89%,and the relative standard deviation was 0.3%~1.1%.The method is simple,rapid,sensitive and accurate.It is suitable for the determination of oxazolidone residues in animal products.