期刊文献+

香蕉含VQ基序蛋白基因MaVQ1的克隆与表达 被引量:3

Cloning and expression analysis of a VQ motif containing protein gene in banana
原文传递
导出
摘要 克隆香蕉含VQ基序蛋白基因MaVQ1,研究其序列特征及其在不同激素处理和逆境胁迫下的表达模式.采用RTPCR技术从‘天宝蕉’中克隆了该基因,对其进行生物信息学分析,并利用实时定量PCR技术(qRT-PCR)研究它在不同组织部位和不同激素、不同逆境胁迫处理下的表达情况.结果显示:MaVQ1编码序列(CDS)长为459 bp,可编码一个分子式为C732H1164N210O207S3、分子量为16314.76、等电点为10.19的不稳定亲水性蛋白.MaVQ1含有保守的VQ结构域,不含跨膜结构和信号肽,与小果野蕉VQ亲缘关系最近.亚细胞定位预测结果显示MaVQ1主要定位在细胞核.蛋白互作预测结果显示MaVQ1与其他香蕉VQ蛋白以及WRKY互作系数最高.启动子顺式作用元件预测结果显示MaVQ1启动子包含多种光响应元件、激素响应元件和逆境胁迫相关作用元件.转录因子结合位点分析结果显示其启动子上存在大量的ERF结合位点.qRT-PCR结果显示:MaVQ1在不同组织部位中的表达无显著差异,其表达受茉莉酸、脱落酸和低温显著诱导,受高温和干旱抑制.本研究表明,与其他植物VQ类似,MaVQ1的表达受多种激素和逆境影响,暗示其可能在香蕉抗逆防御反应过程中发挥着重要调节作用. The aim of this research was to clone and study the sequence of a banana VQ motif-containing protein gene,MaVQ1,and to investigate its expression pattern under different phytohormone and stress treatments.The MaVQ1 gene was cloned from‘Tianbaojiao’bananas by using reverse transcription PCR(RT-PCR)and was subjected to a series of bioinformatic analyses.Its expression patterns in different tissues and organs,and under different treatments,were analyzed using quantitative real-time PCR(qRT-PCR).The coding sequence(CDS)of MaVQ1 is 459 bp,and has been predicted to encode an unstable hydrophilic protein with a molecular formula of C732 H1164 N210 O207 S3,molecular weight of 16,314.76,and isoelectric point of 10.19.The MaVQ1 protein contains a conserved VQ motif,but with no transmembrane structure or signal peptide.Phylogenic relationship analysis revealed that the MaVQ1 protein shared the closest relationship with the VQ protein of Musa acuminata subsp.malaccensis.Protein-protein interaction analysis showed that MaVQ1 shared a high interaction coefficient with other VQ proteins and WRKYs.Protein subcellular localization prediction results showed that MaVQ1 was mainly localized in the nucleus.A variety of light-,hormone-,and stress-responsive cis-acting elements and ERF transcription binding sites were identified in the promoter regions of MaVQ1.Gene expression analysis revealed that the expression of MaVQ1 was stable in different tissues and organs,and its expression could be significantly induced by jasmonic acid,abscistic acid,and low temperature,but suppressed by high temperature and drought treatments.Similar to the VQs from other plant species,MaVQ1 responds greatly to different phytohormones and stress treatments,which suggests that MaVQ1 plays an important regulatory role in banana defense response processes.
作者 刘嘉鹏 刘萍元 孙雪丽 刘范 田娜 伍俊为 程春振 LIU Jiapeng;LIU Pingyuan;SUN Xueli;LIU Fan;TIAN Na;WU Junwei;CHENG Chunzhen(College of Horticulture/Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处 《应用与环境生物学报》 CAS CSCD 北大核心 2021年第4期994-1000,共7页 Chinese Journal of Applied and Environmental Biology
基金 国家重点研发计划项目(2019YFD1000901) 福建农林大学科技创新专项(CXZX2018082) 福建农林大学“校杰出青年科研人才”计划项目(xjq201721)资助 国家现代农业产业技术体系建设专项(CARS-32-11)。
关键词 香蕉 MaVQ1 基因克隆 生物信息学分析 表达模式 banana MAVQ1 gene cloning bioinformatics analysis expression pattern
  • 相关文献

参考文献7

二级参考文献88

  • 1张凤娟,张满良,朱水芳.一种改进的水稻总DNA的快速提取方法[J].植物检疫,2004,18(6):330-332. 被引量:36
  • 2赵世杰,许长成,邹琦,孟庆伟.植物组织中丙二醛测定方法的改进[J].植物生理学通讯,1994,30(3):207-210. 被引量:1164
  • 3康宗利,杨玉红,张立军.植物响应干旱胁迫的分子机制[J].玉米科学,2006,14(2):96-100. 被引量:21
  • 4Andreasson ET,Jenkins J,Brodersen P. The MAP kinase substrate MKS1 is a regulator of plant defense responses[J].European Molecular Biology Organization Journal,2005.2579-2589.
  • 5Chen LG,Zhang LP,Yu DQ. Wounding-induced WRKY8 is involved in basal defense in Arabidopsis[J].Molecular PlantMicrobe Interactions,2010,(05):558-565.
  • 6Cheng WH,Endo A,Zhou L. A unique short-chain dehydrogenase/reductase in Arabidopsis glucose signaling and abscisic acid biosynthesis and functions[J].Plant Cell,2002.2723-2743.
  • 7Cheong JJ,Choi YD. Methyl jasmonate as vital substance in plants[J].Trends in Genetics,2003,(07):409-413.doi:10.1016/S0168-9525(03)00138-0.
  • 8Clarke JD,Volko SM,Ledford HD. Roles of salicylic acid,jasmonic acid,and ethylene in cpr-induced resistance in Arabidopsis[J].Plant Cell,2000,(12):2175-2190.
  • 9Finkelstein RR,Gampala S,Rock C. Abscisic acid signaling in seeds and seedlings[J].Plant Cell,2002,(Suppl):S15-S45.
  • 10Garcia D,Saingery V,Chambrier P. Arnbidopsis haiku mutants reveal new controls of seed size by endosperm[J].Plant Physiology,2003,(04):1661-1670.doi:10.1104/pp.102.018762.

共引文献29

同被引文献24

引证文献3

二级引证文献6

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部