超临界流体色谱分析苯菌灵准确含量的可行性探索

Feasibility Study on the Accurate Determination of Benomyl Using Super-critical Fluid Chromatography

建立了一种可以直接测定苯菌灵原药含量的超临界流体色谱方法。利用超临界流体色谱的特点以及改性剂和添加剂的优化,避免了苯菌灵在仪器分析过程中的降解,通过样品溶剂的选择以及温度的控制来减缓苯菌灵在溶液中降解,最终实现苯菌灵含量的准确测定。该方法可在8 min完成一次样品分析,在2 h的稳定期内,可以利用自动进样器完成12次样品分析。取3份苯菌灵原药,每份样品重复进样2次,苯菌灵峰面积相对标准偏差为1.2%,保留时间相对标准偏差为0.1%。结果表明,本方法可操作性强、分析速度快,测定结果准确,利于在质控实验室实现多样品的自动分析。

A super fluid chromatography method was developed to directly determine the content of benomyl.The degradation within instrument analysis process was avoided by the intrinsic characteristics of super-critical fluid chromatography(SFC)as well as the optimization of modifiers and additives.The degradation of sample solution was also retarded by the sample solvent selection and temperature control,and finally the content of benomyl was determined accurately.The single run was completed in 8 minutes and overall 12 injections could be finished in serial using auto sampler in a stable period of 2 h.The relative standard derivation(RSD)of peak area and retention time of benomyl was 1.2%and 0.1%obtained by three samples with two parallel injections each,respectively.It demonstrated that the developed method was suitable for the automatic sample analysis in QC labs with benefits of easy operation,fast analysis and accuracy.