油棕中果皮MYB转录因子家族的鉴定与表达分析

Identification and Expression Analysis of MYB Transcription Factor Family in Pericarp of Elaeis guineensis Jacq.

油棕(Elaeis guineensis Jacq.)是世界上最重要的油料作物之一,其中果皮含有的棕榈油极其丰富。MYB蛋白是一类广泛存在于真核生物中的转录因子,在植物的生长发育中发挥重要调控作用。本研究基于油棕中果皮转录组的测序结果为基础,利用生物信息学对油棕MYB基因家族进行全面鉴定,并对这些基因的结构、保守域和果实发育不同时期动态表达进行研究,解析油棕MYB转录因子在油棕发育过程中的生物学功能。结果发现,本研究中的38个油棕MYB转录因子,包含26个R2R3-MYB型和8个MYB-related型,以及2个3R-MYB型,不包含4R-MYB类型;MYB蛋白编码约200~700个氨基酸,等电点在4.5~9.5之间;亚细胞定位预测结果显示,所有MYB基因均定位于细胞核中;油棕MYB蛋白具有相似的保守基序组成,38个基因主要分布在13条染色体上,含有1到10个外显子数目;油棕MYB转录因子与多个物种的MYB转录因子具有序列同源性和进化亲缘性;表达模式分析发现,MYB家族基因存在差异表达,且具有几种不同的表达趋势。该研究结果为今后油棕MYB转录因子的脂类代谢途径研究以及功能和调控机制提供重要的研究基础。

Oil palm(Elaeis guineensis Jacq.) is one of the most important oil crops in the world as the mesocarp of the fruit contains extremely rich palm oil. MYB transcription factor family is widely found in eukaryotes and plays an important regulatory role in plant growth and development. Based on the oil palm genome and transcriptome data, MYB gene family was identified by bioinformatics analysis comprehensively, and the structure, conserved domain and tissue specific expression of these genes were also analysis. So as to analyze the biological functions of oil palm MYB transcription factor in the development process of oil palm. The results showed that among the 38 oil palm MYB transcription factors, there were 26 R2 R3-MYB type and 8 MYB-related types, while only 2 3 R-MYB type and no 4 R-MYB type were found. MYB protein encodes about 200~700 amino acids with theoretical pI between 4.5 and 9.5. Subcellular localization showed that all the MYB genes were located in the nucleus. Oil palm MYB protein has similar conserved motifs, 38 genes are mainly distributed on 13 chromosomes, including 1 to 10 exons. Oil palm MYB transcription factors have sequence homology and evolutionary affinity with MYB transcription factors of many species. The expression pattern analysis showed that the MYB family genes were differentially expressed and had several different expression trends. This study provides an important basis for future studies on lipid metabolism pathways, functions and regulatory mechanisms of oil palm MYB transcription factors.