甘蔗割手密种PIN基因家族的鉴定与表达分析

Identification and Expression Analysis of PIN Gene Family in Saccharum spontaneum

PIN (PIN-FORMED)作为生长素输出载体,在生长素极性运输过程中起重要作用,但是目前在甘蔗中还没有相关研究。本研究结合甘蔗割手密种基因组数据、非生物胁迫和生物胁迫下的甘蔗栽培种转录组数据,通过生物信息学分析技术,对甘蔗割手密种PIN基因进行了基因家族鉴定、基因结构及功能分析。分析结果表明,甘蔗割手密种中共有22个SsPIN基因,其中包括15对串联重复基因,启动子包含16类顺式元件,外显子和内含子数目差别明显,分布于14条染色体。SsPINs氨基酸长度在305~791之间,其编码蛋白质分子量在33.20~84.62 kD之间,跨膜结构数目在4~17之间,具有保守基序2,绝大多数属于稳定的碱性蛋白。SsPIN基因家族成员响应低氮、高粱花叶病毒和黑穗病菌胁迫,推测生长素和非生物胁迫及生物胁迫信号通路间存在交叉。本研究为揭示PIN基因在甘蔗逆境适应中的生物学作用奠定了基础,为未来抗性品种开发和选择提供依据。

PIN(PIN-formed), an auxin efflux carrier, plays an important role in polar auxin transport, but there is no PIN-related study on sugarcane until now. In this study, the Saccharum spontaneum PIN genes were identified and their structure and function were analyzed, based on the Saccharum spontaneum genomic data and the sugarcane cultivars transcriptomic data referring to biotic and abiotic stress. The results showed that there were 22 PIN genes in S. spontaneum, which contained a different number of exons and introns, including 15 pairs of tandem repeat genes, and their promoter contains 16 types of cis-elements, distributing on 14 chromosomes. The proteins encoded by SsPINs contain 305~791 amino acid residues and their molecular weights range from 33.20 to 84.62 kD, and the number of transmembrane domains range from 4 to 17. Most of the PIN proteins are stable basic proteins and contain conserved and motif 2. The SsPINs were in response to low nitrogen, Sorghum mosaic virus and Sporisorium scitamineum, suggesting that there was a crosstalk between auxin signal pathway and abiotic or biotic stress signal pathway. This study lays a foundation for revealing the biological role of PIN genes in sugarcane stress adaptation,and provides a basis for the development and selection of resistant varieties in the future.