小桐子miR394家族鉴定及其靶基因的低温表达特性

Identification of Jatropha curcas miR394 Family and the Chilling Expression Characteristics of Its Target Genes

MicroRNA (miRNA)是植物基因转录与翻译的重要调控因子,miR394参与植物多种抗逆性的调节过程。为深入研究miR394在小桐子抗冷性中的调控机制,利用生物信息学方法对小桐子miR394进行鉴定及靶基因预测,并通过实时荧光定量PCR分析了jcu-miR394及其预测靶基因在低温胁迫下的差异表达特性。结果表明,小桐子中共鉴定到两个miR394基因(jcu-MIR394a, jcu-MIR394b),都可形成稳定的茎环结构,成熟体序列都位于茎环结构的5′端,且都为20 nt (5′-UUGGCAUUCUGUCCACCUCC-3′)。靶基因预测与RT-qPCR表达分析显示,小桐子FBXO6是miR394的潜在靶基因,并可能通过miR394b-FBXO6靶向互作参与小桐子抗冷性过程。本研究结果为开展小桐子miR394参与抗冷性机制研究奠定了基础。

MicroRNA(miRNA) is an important gene regulator of plant gene transcription and translation, and miR394 is involved in the regulation of plant resistance. In order to further study the regulation mechanism of miR394 in cold resistance of Jatropha curcas, the identification and target gene prediction of miR394 were carried out by bioinformatics method, and the differential expression characteristics of miR394 and predicted target genes under low temperature stress were analyzed by RT-qPCR. The results showed that two miR394 genes( jcu-MIR394 a, jcu-MIR394 b) were identified from J. curcas, which can formed stable stem-loop structures, and the mature sequences of 20 nt(5′-UUGGCAUUCUGUCCACCUCC-3′) were located at the 5’ end of the stem-loop.Target gene prediction and RT-qPCR expression analysis revealed that FBXO6 was a potential target gene of miR394 and possibly involved in the cold resistance of J. curcas through target interaction of miR394 b-FBXO6.The results might lay a significant foundation for further studies on the cold resistance of miR394 in J. curcas.