稀土纳米复合材料Ce/Ag/ZnO对白念珠菌的氧化应激抗菌机制研究

Study on oxidative stress antibacterial mechanism of rare earth nanocomposites Ce/Ag/ZnO against Saccharomyces albicans

目的 研究稀土纳米复合材料Ce/Ag/ZnO对口腔白念珠菌的氧化应激抗菌机制.方法 通过细菌平板培养、绘制细胞生长曲线、扫描电子显微镜(SEM)观察以及氧化应激相关生化指标检测等实验方法,探究该材料对白念珠菌的抗菌机制.结果 稀土纳米复合材料Ce/Ag/ZnO作用于白念珠菌时,其MIC、MBC分别为0.156 mg/mL、0.313 mg/mL;SEM观察发现细菌皱缩塌陷、内容物泄漏,细胞膜严重破坏;细菌的活性氧(ROS)水平、脂质过氧化(LPO)程度以及过氧化氢酶(CAT)活力值均有明显升高(P<0.05).结论 稀土改性的纳米材料具有良好的抗菌效果,揭示其通过诱导白念珠菌内部产生氧化应激反应的抗菌机制,为未来该材料应用于义齿基托制作,预防口腔义齿性口炎发生提供理论支持.

Objective To study oxidative stress antibacterial mechanism of rare earth nanomaterial Ce/Ag/ZnO against oral Saccharomyces albicans.Methods Bacterial plate culture,bacterial growth curve drawing and scanning electron microscopy(SEM)and oxidative stress related biochemical indexes detection and other experimental methods were adopted.Results The MIC and MBC of Ce/Ag/ZnO on Saccharomyces albicans were 0.156 mg/mL and 0.313 mg/mL,respectively.SEM showed that the bacteria crumpled and collapsed;the contents leaked;the cell membrane was seriously damaged.The level of reactive oxygen species(ROS),the degree of lipid peroxidation(LPO)and the activity value of catalase(CAT)of bacteria were significantly increased(P<0.05).Conclusion This experiment proves that nano-materials modified by rare earth still have good antibacterial effect,and reveals their antibacterial mechanism of inducing oxidative stress reaction inside saccharomyces albicans.This provides theoretical support for the application of this material in the manufacture of denture base and for the prevention of denture stomatitis in the future.