康柏西普对晶状体上皮细胞增殖的抑制作用及其相关机制

Inhibitory effect of conbercept on the proliferation of lens epithelial cells and related mechanism

目的探讨康柏西普(conbercept)对晶状体上皮细胞(LECS)增殖的抑制作用及其相关机制,为临床预防和治疗后发性白内障提供实验依据。方法实验研究。实验分为5组,即对照组(0 mg/ml)和4个不同浓度的康柏西普组,康柏西普的浓度分别为(0.1 mg/ml,0.5 mg/ml,1.0 mg/ml,2.0 mg/ml),用不同浓度的康柏西普分别处理培养的人晶状体上皮细胞株SRA01/04,用MTT法检测康柏西普对晶状体上皮细胞存活率的影响,用流式细胞仪检测细胞凋亡情况,用western blot法检测细胞凋亡相关蛋白Bax,Bcl-2的表达水平。结果随着康柏西普浓度的增加,细胞的存活率逐渐降低,与对照组相比,0.1 mg/ml组的细胞存活率降低无统计学意义(P>0.05),0.5 mg/ml组、1.0 mg/ml组、2.0 mg/ml组的细胞存活率降低均有统计学意义(P<0.05);细胞凋亡率随着药物浓度的增加逐渐升高,各组与对照组相比均具有统计学意义(P<0.05);细胞促进凋亡蛋白Bax表达水平升高,其中0.1 mg/ml组与对照组相比无统计学意义(P>0.05),0.5 mg/ml组,1.0 mg/ml组,2.0 mg/ml组与对照组相比有统计学意义(P<0.05);细胞抑制凋亡蛋白Bcl-2的表达水平逐渐降低,各组与对照组相比均具有统计学意义(P<0.05)。结论一定浓度范围的康柏西普能够促进晶状体上皮细胞的凋亡、抑制晶状体上皮细胞的增殖。

Objective To observe the inhibitory effect of conbercept on the proliferation of lens epithelial cells(LECs)and its related mechanism,to provide experimental basis for clinical prevention and treatment of posterior capsule opacification(PCO).Methods The experiment was divided into five groups,including the control group and four different concentration of conbercept groups(0.1 mg/ml,0.5 mg/ml,1.0 mg/ml,2.0 mg/ml).Different concentrations of conbercept were used to culture human LECs lines SRA01/04.The survival rate of cell was detected by the thiazolam(MTT)assay,the apoptosis was detected by the flow cytometry,and the expression levels of apoptosis-related proteins Bax,and Bcl-2 were detected by Western Blot(WB).Results With increasing concentration of conbercept,the survival rate of cells gradually decreased.Compared with the control group,the survival rate of cells in the 0.1 mg/ml group was not statistically significant(P>0.05),while the cell survival rate in the 0.5 mg/ml group,1.0 mg/ml group and the 2.0 mg/ml group decreased statistically(P<0.05).The apoptosis rate gradually increased with increasing drug concentration,which was significantly different compared with the control group(P<0.05).The expression level of apoptosis-promoting protein Bax increased,and was not significantly different between the 0.1 mg/ml group and the control group(P>0.05),but the expression level in the 0.5 mg/ml group,1.0 mg/ml group and the 2.0 mg/ml group was statistically significantly different compared with the control group(P<0.05).The expression level of cytostatic protein Bcl-2 gradually decreased,and each group was significantly different compared with the control group(P<0.05).Conclusions Conbercept in a certain concentration range can promote the apoptosis of LECs and inhibit the proliferation of LECs.