口蹄疫病毒O型泛亚株VP1蛋白的二级结构及抗原表位预测

Prediction of the Secondary Structure and Epitopes of VP1 Protein of FMDV-O PanAsia Strain

为获得口蹄疫病毒(FMDV)O/PanAsia毒株VP1蛋白的抗原信息,从而为制备多肽疫苗提供理论依据,运用生物信息学软件,对O/PanAsia毒株的结构蛋白VP1理化性质、结构功能以及细胞表位进行分析,预测抗原表位以选择合适肽段。应用ProParam、TMHMM Server、ProScale和SignaIP等在线工具,分析VP1蛋白氨基酸序列,运用计算机技术和分子生物学软件,分析预测VP1蛋白的基本理化性质、结构功能以及可能的B细胞和T细胞抗原表位,筛选B细胞表位肽段并对VP1的两个主要T细胞表位CTL和Th细胞表位进行预测。结果显示:O/PanAsia毒株VP1蛋白的等电点为9.49,相对分子质量为23520.83;VP1蛋白的B细胞表位为8~23、135~149和193~205位氨基酸。预测该VP1有10个CTL和10个Th细胞抗原表位,表明其具有较好的免疫原性;最终筛选出VP1蛋白的5个CTL和5个Th优势表位。用生物信息学方法预测O/PanAsia毒株VP1蛋白为稳定性蛋白,含有T、B淋巴细胞抗原表位。本研究为FMDV的进一步研究和疫苗制备奠定了基础。

In order to collect the antigen information of VP1 protein of foot-and-mouth disease virus type O(FMDV-O)PanAsia strain,and to provide a theoretical basis for preparation of peptide vaccines,the physical and chemical properties,structural functions and cell epitopes of the structural protein VP1 of O/PanAsia strain were analyzed by the bioinformatics software to predict antigenic epitopes and subsequently select an appropriate peptide.The amino acid sequence of VP1 protein was analyzed by use of ProParam,TMHMM Server,Pro Scale,SignaIP and other online tools.The basic physical and chemical properties,structural functions and possible B-cell and T-cell antigenic epitopes of VP1 protein were analyzed and predicted by the computer technology and molecular biology software,followed by selection of B-cell epitope peptides and prediction of two main T-cell epitopes including CTL and Th.It was found that the isoelectric point of VP1 protein was 9.49,the relative molecular mass was 23520.83,and the B-cell epitopes of VP 1 protein were amino acids 8 to 23,135 to 149,and 193 to 205.It was predicted that 10 CTL and 10 Th cell epitopes were contained in the VP1 protein that was with good immunogenicity;then 5 CTL and 5 Th dominant epitopes were selected.The VP1 protein of O/PanAsia strain was predicted as a stable protein by the bioinformatics methods,which contained T and B lymphocyte antigenic epitopes.A basis was provided for further study on FMDV and development of vaccine s.