白花鬼针草中氨基酸提取工艺优化研究

A Study on Optimization of the Extraction Process of Amino Acids from Bidens Alba

为探讨白花鬼针草中游离氨基酸的最佳提取工艺条件,通过单因素试验和正交优化的方法,考察各因素(乙醇体积分数、料液比、提取次数、提取时间等)对氨基酸提取率的影响。研究结果显示,乙醇体积分数对氨基酸提取率影响最大。优化所得最佳提取工艺条件为:乙醇体积分数为60%、料液比为1∶40、提取次数3次和提取时间为40 min。该提取工艺可为后续进一步开发鬼针草资源提供参考依据。

In order to study the optimal extraction process conditions of free amino acids in Bidens alba,the single factor experiment and orthogonal optimization have been applied,so as to analyze the impact of various factors(including the ethanol volume fraction,solid-liquid ratio,extraction times and extraction time)on the extraction rate of amino acids.As indicated in the study results,the ethanol volume fraction has the greatest impact on the extraction rate of amino acids.With optimization,the optimal extraction process conditions are as follows:the ethanol volume fraction will be 60%,solid-liquid ratio 1∶40,extraction times 3 and extraction time 40 minutes.The extraction process may provide certain reference for further development of Bidens alba.