氟氯氰菊酯残留检测与紫外降解荧光光谱研究

Detection and investigation of cyfluthrin pesticide residue on UV degradation by using fluorescence spectroscopy

氟氯氰菊酯杀虫效果良好,应用广泛,而其农药残留会对环境与食品安全带来潜在的影响,因此氟氯氰菊酯残留检测及其有效降解具有重要的社会意义。通过荧光分光光度计对氟氯氰菊酯进行了荧光光谱实验研究,发现296 nm处为特征峰。根据光谱图对不同的氟氯氰菊酯浓度值与相应特征峰荧光强度值进行回归分析建模,得到氟氯氰菊酯农药残留检测模型函数,相关系数R为0.9978,回收率在96%~104%范围内,平均回收率为98.9%,相对标准偏差为2.6%。基于紫外光照射对氟氯氰菊酯进行降解,获取降解后的药液荧光光谱,得到降解时间与特征峰荧光强度之间的降解模型函数,相关系数R为0.9932,平均回收率为98.25%。结合所得氟氯氰菊酯农药残留检测模型函数及其降解率公式,进一步计算得到氟氯氰菊酯经过不同降解时间后对应的降解率值。当降解11 min后,降解率为72.6%,降解80 min后,降解率为97.8%。实验表明,荧光光谱能够有效检测氟氯氰菊酯农药残留,而且紫外光照射可以有效降解氟氯氰菊酯。

Cyfluthrin is widely used due to its good insecticidal effect,but its residual is harmful to the environment and food.Therefore,the detection of cyfluthrin pesticide residue and investigation of its efficient degradation are important.Fluorescence spectra of cyfluthrin at different concentrations are recorded by fluorescence spectrophotometer.The results show that the fluorescence spectroscopy characteristic peak of cyfluthrin is 296nm.Using the regression analysis,and the equation of predictive model between the concentration of cyfluthrin and the intensity of characteristic peak was obtained(R=0.9978).The recovery was in the range of 96%~104%,the average recovery was 98.9%,and the relative standard deviation was 2.6%.Ultraviolet(UV)irradiation was used to degrade the cyfluthrin solution.The equation of predictive model between the intensity of fluorescence spectra of cyfluthrin solution after degradation and the degradation time was obtained(R=0.9932),the average recovery was 98.25%.Combined with the model function of cyfluthrin pesticide residue detection and its degradation rate formula,the corresponding degradation rate value of cyfluthrin after different degradation time is further calculated.For example,the degradation rate was 72.6%after 11 minutes,while it reached 97.8%after 80 minutes.Therefore,the cyfluthrin residue can be effectively detected by fluorescence spectra,and the cyfluthrin can be efficiently degraded by UV treatment as well.