柴术理胃饮对肝郁脾虚型功能性消化不良大鼠血清MTL、GAS的影响

Effect of CZLWY on Serum MTL and GAS in FD Rats with Liver Depression and Spleen Deficiency

目的:研究柴术理胃饮对肝郁脾虚型功能性消化不良(Functional dyspepsia,FD)大鼠血清中胃动素(MTL)与胃泌素(GAS)水平的影响。方法:60只SPF级雄性SD大鼠随机分为正常组、模型组、西药组和中药低、中、高剂量组,每组10只。除正常组外,其余各组采用复合因素刺激(慢性疲劳+悬尾+饮食失节)方法制备FD模型。造模成功后给予各组药物干预。中药组给予柴术理胃饮,其中低、中、高剂量组给药剂量分别为13.5、27、54 g/kg;西药组给予多潘立酮3.125 mg/kg;正常组、模型组给等量0.9%氯化钠注射液。每日给药1次,21 d后观察大鼠胃排空率和小肠推进率、胃窦病理形态变化及血清MTL、GAS表达水平。结果:与正常组相比,模型组大鼠胃排空率和小肠推进率明显下降(P<0.05),血清中MTL、GAS水平均有不同程度降低(P<0.05);与模型组相比,中药各组大鼠的胃排空率和小肠推进率均有不同程度的提高(P<0.05),血清MTL、GAS水平不同程度升高(P<0.05);与中药中剂量组、高剂量组比较,低剂量组大鼠胃排空率明显降低(P<0.05);中药各剂量组与西药组MTL水平含量升高(P<0.05);中药各剂量组与西药组GAS水平升高,与低剂量组比较,高剂量组GAS明显升高(P<0.05)。结论:柴术理胃饮可以促进肝郁脾虚型FD大鼠的胃蠕动功能,缩短小肠推进时间,提高血清MTL、GAS水平,这可能是其治疗肝郁脾虚型FD的机制之一。

Objective:To study the effects of Chaizhu Liwei Yin(CZLWY)on serum levels of motilin(MTL)and gastrin(GAS)in patients with functional dyspepsia(FD)due to Liver depression and Spleen deficiency.Methods:60 SPF healthy male SD rats were randomly divided into the normal group,the model group,the CZLWY groups of low-dose(13.5 g/kg),medium-dose(27 g/kg)and high-dose(54 g/kg),as well as the Doperidone group,with 10 rats in each group.FD model was prepared by compound factors stimulation(chronic fatigue,tail suspension and irregular diet).After 21 days of the interventions,the general conditions of the rats were observed and recorded,the gastric emptying rate and the small intestinal propulsive rate were calculated,the pathological changes of gastric antrum were observed by HE staining,and the serum levels of MTL and GAS were detected by ELISA.Results:Compared to those in the normal group,the gastric emptying rate and the small intestinal propulsive rate were significantly decreased(P<0.05),and the serum levels of MTL and GAS were decreased in different degrees in the model group(P<0.05).Compared to those in the model group,the gastric emptying rate and the small intestinal propulsive rate were increased(P<0.05),and the serum levels of MTL and GAS were also increased in the CZLWY groups(P<0.05).In terms of the gastric emptying rate,it was significantly decreased in the low-dose group compared to that in the medium-dose group and the highdose group(P<0.05).In terms of the level of MTL,it was increased in the CZLWY groups and in the Doperidone group.In terms of the level of GAS,it was increased in the CZLWY groups and in the Doperidone group;compared to that in the low-dose group,it was significantly increased in the high-dose group(P<0.05).Conclusion:CZLWY can promote gastric peristalsis function,shorten small intestinal propulsive time,and increase the serum levels of MTL and GAS in FD rats with Liver depression and Spleen deficiency,which may be one of the mechanisms of CZLWY in the treatment of FD with Liver depression and Spleen deficiency.