饮水型地方性氟中毒病区儿童口腔卫生状况及其肠道菌群特征研究

Study on oral hygiene status and intestinal flora characteristics of children in drinking-water-borne endemic fluorosis areas

目的探究饮水型地方性氟中毒病区儿童口腔卫生状况,并对其肠道菌群特征进行分析。方法选择2019年1月-2019年12月商丘市疾控中心收集1000例学龄期儿童作为试验对象,采用电极法对水氟进行测定,选择Dean/s法诊断氟斑牙,并对饮水型地方性氟中毒病区儿童口腔卫生状况以及肠道菌群特征进行分析。结果1000名学龄儿童中,氟斑牙发生人数为100例,所占比10.00%,疾病程度中轻度所占比50.00%、中度所占比20.00%、重度所占比30.00%;氟斑牙指数为(0.24±0.03)、水氟水平(0.85±0.21)mg/L、尿碘水平(210±25.36)μg/L;结果显示,肠道酸杆菌门的存在、Thermi菌门的缺失、消化球菌属、普氏菌属、Dehalobacterium菌属丰度的增加、链球菌属、瘤胃球菌属丰度的降低与儿童氟斑牙存在密切关联性。从儿童氟斑牙年龄分布情况来看,氟斑牙一般发生在年龄在11岁左右儿童中,其次为12岁儿童,不同年龄段儿童氟斑牙发生率相比,差异有统计学意义,P<0.05。结论饮水型地方性氟中毒病区儿童口腔卫生情况较差,并且肠道菌群特征呈多样性。

Objective To explore the oral hygiene status of children in drinking-water-borne endemic fluorosis areas and analyze the characteristics of intestinal flora.Methods From January 2019 to December 2019,1000 school-age children were collected from the Shangqiu City Center for Disease Control and Prevention as the test subjects.The electrode method was used to determine the water fluoride,the Dean/s method was selected to diagnose dental fluorosis,and the drinking water-type endemic The oral hygiene status of children in fluorosis areas and the characteristics of intestinal flora were analyzed.Resu Its Among 1000 school-age children,100 cases of dental fluorosis occurred,accounting for 10.00%,and the disease degree showed 50.00%mild cases,20.00%moderate cases and 30.00%severe cases.Dental fluorosis index,water fluoride level and urine iodine level were(0.24±0.03),(0.85±0.21)mg/L and(210±25.36)μg/L respectively.Results displayed that the presence of Acidobacter entericus,the lack of Thermi phylum,increases of Peptococcus,Prevotella and Prevotella and Dehalobacterium abundance and the decreases of abundance of Streptococcus and Ruminococcus were closely related to dental fluorosis in children.From the perspective of the age distribution of dental fluorosis in children,dental fluorosis generally occurred in children around 11 years old,followed by 12-year-old children.There was a statistically significant difference in the incidence rate of dental fluorosis among children of different ages.P<0.05.Conclusion Children in drinking-water-borne endemic fluorosis areas have poor oral hygiene,and the characteristics of intestinal flora are diverse.