金龟子绿僵菌CQMa421杀蟑饵剂药效测定与现场应用研究

Efficacy of Metarhizium anisopliae CQMa421 bait against cockroaches:A laboratory and field study

目的测试金龟子绿僵菌CQMa421饵剂室内和现场防治德国小蠊效果,为真菌杀虫剂在防治卫生害虫上的应用提供参考。方法配制不同含量的金龟子绿僵菌CQMa421饵剂,分别在实验室和现场环境研究其对德国小蠊种群数量的影响,采用SPSS 25.0软件对死亡率、半数致死时间(LT_(50))、半数致死浓度(LC50)和90%致死浓度(LC90)进行分析,评价金龟子绿僵菌CQMa421饵剂杀蟑效果;采用χ^(2)检验分析不同组别间死亡率的差异。结果室内实验结果表明,1×10^(8)孢子/g金龟子绿僵菌CQMa421饵剂喂食德国小蠊后12 d,其LT_(50)均值为(6.85±0.24)d;现场实验中,采用每2.5 m^(2)放置1盒1×10^(8)孢子/g的金龟子绿僵菌CQMa421饵剂,药剂投放30 d后,相对密度下降率为93.22%。结论 1×10^(8)孢子/g的金龟子绿僵菌CQMa421饵剂可有效控制德国小蠊种群密度。

Objective To evaluate the effects of the Metarhizium anisopliae CQMa421 bait in controlling Blattella germanica in both laboratory and field settings,and to provide a reference for the application of fungal insecticides in the control of hygiene pests.Methods The M.anisopliae CQMa421 baits at different concentrations were prepared,and their effects on the population density of B.germanica were measured in the laboratory and field settings.SPSS 25.0 software was used to analyze mortality,median lethal time(LT_(50)),median lethal concentration(LC50),and 90% lethal concentration(LC90) to evaluate the cockroach-killing effects of the M.anisopliae CQMa421 baits.The chi-square test was used to compare mortality between groups.Results The laboratory test showed that after feeding B.germanica for 12 days with the M.anisopliae CQMa421 bait at 1×10^(8) spores/g,LT_(50) was(6.85±0.24) days.In the field test,after placing the M.anisopliae CQMa421 bait at 1×10^(8) spores/g(1 box/per 2.5 m^(2)) for 30 days,the relative density of B.germanica decreased by 93.22%.Conclusion The M.anisopliae CQMa421 bait at 1×10^(8) spores/g can effectively control the population density of B.germanica.