摘要
《生物化学与分子生物学综合实验》是由北京师范大学面向生物学专业本科生开设的,集技能训练与创新能力培养于一体的大规模开放的在线课堂。该课程以大肠杆菌碱性磷酸酶贯穿始终,从基因的克隆到蛋白质的表达纯化以及酶学特性的研究,还包括碱性磷酸酶抗体的制备和效价测定等,让学生能够完整地、系统地学习和掌握常用的生物化学与分子生物学实验技术。大肠杆菌碱性磷酸酶是一个非常理想的实验研究对象,从基因到蛋白质都有经典的科研文献报道。在教学实践过程中,将这些文献资源作为重要的辅助教学资源,可以帮助教师和学生进行探究式的学习,形成理性批判性的科学态度,同时也为教师和学生提供了更严谨与更个性化的帮助,显著地提升了教学质量和学生的科研素质,在一流课程建设中发挥着重要的作用。
Comprehensive Laboratory of Biochemistry and Molecular Biology is a compulsory open online course that integrates lab skill training and innovation ability training for undergraduates majoring in biology in Beijing Normal University. This course focuses on alkaline phosphatase, including cloning of the Escherichia coli alkaline phosphatase gene, expression and purification of the protein, research on its enzymatic characteristics, preparation of its antibody and determination the titers of the antibody, etc. The comprehensive lab course covers from the gene to the protein, so that students can learn and master commonly used biochemical and molecular biology experimental techniques systematically. Escherichia coli alkaline phosphatase is an ideal experimental research object. And there are very classic and systematic scientific research literature reports. As important auxiliary teaching means, these literature resources can help teachers and students to carry out learning inquiries, form rational and critical scientific attitudes and spirits, and also greatly improve the quality of teaching and students′ scientific research quality, which plays an important role in the construction of first-class course of Comprehensive Laboratory of Biochemistry and Molecular Biology.
作者
尹燕霞
杨冬
佟丽
李森
向本琼
骆静
YIN Yan-Xia;YANG Dong;TONG Li;LI Sen;XIANG Ben-Qiong;LUO Jing(Department of Biochemistry and Molecular Biology,College of Life Sciences,Beijing Normal University,Beijing 100875,China)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2021年第10期1408-1414,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
北京市教改项目和国家自然科学基金(No.82074056)资助。
关键词
大规模开放的在线课堂
经典文献阅读
综合实验
分子生物学
碱性磷酸酶
Massive Open Online Course(MOOC)
reading of classic literature
comprehensive experiment
molecular biology
alkaline phosphatase(AKP)