冻-放射复合伤中大鼠肺损伤情况及其机制研究

Mechanism of lung injury induced cold-radiation

目的探讨冻-放射复合伤中大鼠肺损伤情况及其可能的机制。方法将40只雄性大鼠随机分成对照组、寒冷组、放射组和冻-放射组,每组各10只。通过检测大鼠的生命体征、血常规、炎性因子等评价大鼠损伤程度。HE染色观察肺组织病理学改变。蛋白免疫印迹法检测氧化应激、凋亡相关蛋白表达,并探讨肺损伤的相关机制。结果与对照组比较,放射组、冻-放射组大鼠体质量明显降低,且冻-放射组低于放射组,差异有统计学意义(P<0.05)。与对照组比较,寒冷组、放射组和冻-放射组大鼠白细胞、淋巴细胞、SOD-2、Bcl-2表达均明显降低,大鼠肺重量/体质量比值、湿重/干重比值、MDA5、Cytochrome C、Bax、Ahsg、RAGE、NF-κB蛋白表达明显升高,差异有统计学意义(P<0.05)。与对照组比较,放射组、冻-放射组大鼠的pH值明显降低,动脉二氧化碳分压、血红蛋白明显升高,差异有统计学意义(P<0.05)。寒冷组、放射组和冻-放射组大鼠血清中白细胞介素1、转化生长因子β可表达水平显著高于对照组,且冻-放射组高于寒冷组和放射组,差异有统计学意义(P<0.05)。结论冻-放射复合伤可导致大鼠急性肺损伤、氧化应激和凋亡,其机制可能与Ahsg/RAGE/NF-κB信号通路相关。

Objective To investigate the lung injury in combined freezing and radiation injury and elucidate its possible mechanism.Methods Forty male rats were randomly divided into control group, cold group, radiation group and freeze radiation group, with 10 rats in each group.The degree of injury was evaluated by detecting vital signs, blood routine and inflammatory factors.The pathological changes of lung tissue were observed by HE staining.Western blot was used to detect the expression of oxidative stress and apoptosis related proteins, and to explore the mechanism of lung injury.Results Compared with the control group, the body mass of rats in the radiation group and freeze radiation group decreased significantly, and the body mass of rats in the freeze radiation group was lower than that in the radiation group(P<0.05).Compared with the control group, the expressions of white blood cells, lymphocytes, SOD-2 and Bcl-2 in cold group, radiation group and frost-radiation group were significantly decreased, and the lung weight/body weight ratio, wet weight/dry weight ratio, MDA5,Cytochrome C,Bax, Ahsg, RAGE,NF-κB protein expression were significantly increased, and the difference was statistically significant(P<0.05).Compared with the control group, the pH value of rats in the freeze-radiation group was significantly decreased, and the arterial partial pressure of carbon dioxide and hemoglobin were increased, with statistical significance(P<0.05).The expression levels of interleukin 1(IL-1) and transforming growth factor β(TGF-β)in serum of rats in cold group, radiation group and freeze-radiation group were significantly higher than those in control group, and the freeze-radiation group was higher than that in cold group and radiation group, the difference was statistically significant(P<0.05).Conclusion Freeze radiation combined injury leads to acute lung injury, oxidative stress and apoptosis in rats, and its mechanism may be related to Ahsg/RAGE/NF-κB signal path related.