lnc-005028序列及其在鹅肥肝形成中的作用

Sequence of lnc-005028 and its function in the formation of goose fatty liver

为了研究lnc-005028在鹅肝中的具体序列和功能,以四川白鹅为研究对象,通过克隆发现lnc-005028全长1066 bp。荧光定量RT-PCR分析表明lnc-005028在正常鹅肝脏中特异性高表达,极显著高于其他组织(P<0.01);填饲后肝脏中lnc-005028表达水平与皮脂中无显著差异(P>0.05)。0.2、0.4和0.6 mmol/L的棕榈酸处理鹅原代肝细胞能极显著影响lnc-005028的表达(P<0.01),且其表达水平的变化趋势与半胱氨酸蛋白酶3(caspase-3)相似。提示:lnc-005028的作用可能与鹅原代肝细胞凋亡有关,为进一步研究鹅肝中lnc-005028的功能提供了科学依据。

The specific sequence and function of lnc-005028 in goose liver were studied here,using Sichuan White goose as experimental subjects.The results were as follows:the result of cloning showed that lnc-005028 presented a total length of 1066 bp and the result of qRT-PCR showed that its specific expression was significantly higher in the tissues of goose liver than in the other metabolic tissues(P<0.01).This expression was similar to that in sebum after overfeeding.Besides,palmitic acid had significant effect on the expression of lnc-005028 in the primary hepatocytes of goose at doses of 0.2,0.4 and 0.6 mmol/L(P<0.01),and trend in changes of the expression was similar to that of caspase-3.This result suggested that the role of lnc-005028 was related to apoptosis of primary hepatocytes on gooses,which was a scientific reference for further research on the functional verification of lnc-005028 in goose liver.