miR-23-3p及靶基因TIGIT在寻常性银屑病患者外周血单个核细胞的表达

Expression of miR-23-3p and its target gene TIGIT in PBMC of the patients with psoriasis vulgaris

目的研究microRNA(miRNA)及其靶基因在寻常性银屑病患者与健康人外周血单个核细胞(PBMC)中的表达谱差异。方法利用miRNA表达谱芯片结合生物信息学分析银屑病患者和健康对照组PBMC差异表达的miRNA,筛选可能核心基因并进行验证。结果筛选出268个差异表达的miRNA,其中有10个明显上调,41个明显下调,miR-23-3p等11个具有统计学差异,筛选靶基因为TIGIT。结论差异表达的miRNA可能通过调控靶基因参与多种信号通路,促进银屑病的发生发展。

Objective To investigate the expression of miRNA and its target gene in the peripheral blood mononuclear cell(PBMC) of the patients with psoriasis vulgaris and healthy people. Methods The miRNA microarray combined with bioinformatics were used to analyze the differentially expressed miRNAs in PBMCs of the patients with psoriasis and healthy controls, meanwhile the possible target gene was screened. Results A total of 268 differentially expressed miRNAs were screened out, among which 10 were significantly upregulated, 41 were significantly down-regulated, and there were significant differences in the expression of 11 miRNAs including miR-23-3 p. The target gene was selected as T cell immunoglobulin and ITIM domain(TIGIT). Conclusion Differentially expressed miRNAs may promote the occurrence and development of psoriasis by regulating target genes and participating in multiple signal pathways.