感染赤星病后烟草叶片的蛋白质表达差异

Differential Expression of Proteins in Leaves of Different Tobacco Varieties Infected by the Pathogen of Tobacco Brown Spot

为了解烟草受赤星病菌侵染后蛋白质表达变化情况,探讨不同抗性烟草品种的差异,对“云烟87”“K326”和“Beinhart1000-1”的叶片进行了人工接种,通过双向电泳比较分析感染前和感染后第6 d烟草叶片蛋白差异点.结果表明,通过双向电泳共有148个表达差异明显的蛋白质点,选取差异点进行质谱分析,通过质谱结果及数据库搜索鉴定,共成功鉴定到38个差异蛋白质点.经过分析,根据这38个蛋白所参与的代谢途径和生化功能将它们归纳成5个类群:碳代谢途径相关的蛋白质、光合作用相关的蛋白质、能量代谢相关的蛋白质、防御相关的蛋白质和蛋白合成相关的蛋白质,同时还有5个未知蛋白.

In order to understand the changes in protein expression after tobacco brown spot infection and investigate the differences of different tobacco cultivars in disease resistance,three tobacco cultivars(‘Yunyan 87’,‘K326’and‘Beinhart1000-1’)were artificially inoculated with the pathogen of tobacco brown spot,and the protein levels at 6 days before and after infection were investigated with two-dimensional electrophoresis(2-DE).Using 2-DE and image analysis system,148 significantly different expression protein spots were detected.The differentially expressed proteins were analyzed by mass spectrometry.A total of 38 differentially expressed protein spots were identified by MALDI-TOF/TOF MS analysis and database searching.Based on the metabolic pathways and biochemistry functionals,we grouped them into 6 clusters:carbon metabolism pathway-related proteins,photosynthesis-related proteins,energy metabolism-related proteins,defensive response-related proteins,protein synthesis-related proteins and 5 uncharacterized proteins.The results of this study suggested that differently expressed proteins might play an important role in functional cloning of resistance-related genes in tobacco.