生活污水对湿地光合细菌种类和丰度的影响——以马鞍溪湿地公园为例

The Effect of the Discharge of Domestic Sewage on the Community Structure and Abundance of Wetland Photosynthetic Bacteria——Taking Maanxi Wetland Park as an Example

为了研究马鞍溪湿地光合细菌群落结构和多样性,以及生活污水排放对其的影响,克隆了光合细菌保守的标记基因pufM、构建文库和测序,比较有或无生活污水排放样点淤泥中光合细菌的种类和丰度.研究发现两者分别有151种和152种光合细菌,其中76%的光合细菌相同.但是,两者的细菌群落结构显著不同.生活污水排放后在种水平上明显增多的是胶状红长命菌(Rubrivivax gelatinosus)和湖栖菌(Limnohabitans sp.Hippo3),明显减少的有水生甲基杆菌(Methylobacterium aquaticum),能够去除亚硝氮的不产氧光合细菌(Anoxygenic Phototrophic Bacteria,APB)-紫色海洋着色菌(Marichromatium purpuratum)和柠檬色微菌(Citromicrobium sp.JL477).这揭示了马鞍溪湿地光合细菌群落结构以及生活污水排放对光合细菌种类和群落结构的影响.

To explore the community structure and diversity of photosynthetic bacteria in Maanxi wetland and the effect of the discharge of domestic sewage,pufM biomarker gene for photosynthetic bacteria was used to clone and construct library for sequencing.The species and abundance of photosynthetic bacteria in the mud samples collected at the sites with and without domestic sewage discharge were compared.We found 151 and 152 OTUs(operational taxonomic units),respectively,from the two sites,76%of which(115 OTU)the two sites had in common.However,the bacteria community structure of the two sites was significantly different.The discharge of domestic sewage significantly increased the abundance of Rubrivivax gelatinosus and Limnohabitans sp.Hippo3 and significantly decreased the abundance of Methylobacterium aquaticum,anoxygenic phototrophic bacteria-Marichromatium purpuratum and Citromicrobium sp.JL477.