罕见B(A)亚型的血清学特征及基因序列分析

Serological characteristics and gene sequence analysis of rare B(A)subgroup

目的:分析罕见的B(A)亚型血清学特征及基因测序结果。方法:选取2013年3月至2021年6月温州医科大学附属第二医院的8例ABO血型正反定型不一致或抗原减弱的标本,分别采用序列特异性引物-聚合酶链反应(PCR-SSP)法检测ABO基因分型,直接测序法进行基因测序分析,聚凝胺法与A型、B型、AB型、O型供血者进行交叉配血。结果:8例标本血清学正定型结果表现为A弱B型,基因测序表现为B(A)型,其中基因型B(A).04/O.01.022例、B(A).02/O.01.022例、B(A).04/O.01.012例、B(A).04/A.01.011例、B(A).02/O.01.011例;与O型、B型供血者交叉配血主测均阴性,与AB型供血者交叉配血次测阴性。结论:罕见的B(A)亚型基因型以B(A).04、B(A).02为主,其血清学表现特征“A弱B强”。

Objective:To analyze the serological characteristics and gene sequencing results of rare B(A)subgroup.Methods:Eight specimens with inconsistent positive and negative ABO typing or antigen weakening were collected from the Second Affiliated Hospital of Wenzhou Medical University from March 2013 to June 2021.Polymerase chain reaction sequence-specific primer(PCR-SSP)was used to detect ABO genotyping,direct sequencing method for gene sequencing,polybrene method with type A,B,AB and O donors for cross matching.Results:Eight samples showed AweakB type in routine serological results and B(A)type in gene sequencing,including 2 cases of B(A).04/O.01.02,2 cases of B(A).02/O.01.02,2 cases of B(A).04/O.01.01,1 case of B(A).04/A.01.01,and 1 cases of B(A).02/O.01.01.Main side cross matching with O and B donors was negative,so as the secondary side of cross matching with AB donors.Conclusion:The genotype of rare B(A)subgroup was mainly B(A).04 and B(A).02,with its serological characteristics of“A is weak and B is strong”.