强直性脊柱炎的脂质组学研究

Study of ankylosing spondylitis on pipidomics of the human serum

目的通过质谱联用技术对强直性脊柱炎(ankylosing spondylitis,AS)患者的血清进行脂质组学研究,寻找潜在的AS脂质生物标志物。方法采用超高效液相色谱-三重四级杆质谱(UPLC/MS/MS)联用技术,对61例AS患者及28例正常人的血清样本进行脂质组学研究,比较AS患者与正常人血清中的差异脂质代谢物。结果AS组和健康对照组(C组)的血清脂质指纹图谱存在明显差异,其中差异较明显的脂类代谢物主要是溶血性甘油磷脂酰胆碱类(Lyso-GPC)和甘油三酯类(TG);差异明显的脂类代谢物共定性鉴别出21种,与C组相比这21种差异脂质在AS组患者血清中共同表现了下降趋势,其中以TG类最为明显。结论以TG(54∶7)、Lyso-GPC(18∶2)为代表的21种差异脂质代谢物可能是AS的特异性脂质代谢标志物,可以为AS的临床研究提供进一步的理论基础。

Objective:To study the lipidomics characteristics of ankylosing spondylitis(AS)by MS for the disease lipid markers.Methods:The serum samples of 61 AS patients and 28 normal persons were studied by using the technique of ultra-high performance liquid chromatography triple quadrupole mass spectrometry(UPLC/MS/MS),and the serum lipid metabolites of patients with AS and healthy people were compared.Results:Serum lipid fingerprint of AS patients and the healthy people were significantly different,and the obvious differences in lipid metabolites were lysophosphatidylcholine(Lyso-GPC)and glycerin trilaurate(TG);21 kinds of lipid metabolites with obvious difference were identified.Compared with the healthy volunteers,these 21 kinds of different lipids showed a downward trend in the serum,among which TG were the most obvious.Conclusion:The 21 different lipid metabolites represented by TG(54∶7)、Lyso-GPC(18∶2)may be the lipid metabolism markers of AS,which can provide further theoretical basis for the clinical research of AS.