近红外七甲川菁染料IR-783在宫颈癌诊断中的作用研究

Role of near-infrared fluorescence imaging heptanine dye IR-783 in the diagnosis of cervical cancer

目的:探讨近红外七甲川菁染料IR-783作为检测宫颈癌的显像剂的价值以及IR-783介导成像的潜在机制。方法:以宫颈癌细胞为研究对象,观察IR-783染料的摄取、积累和亚细胞定位。对患有宫颈癌异种移植瘤的小鼠和新采集的临床宫颈癌标本进行全身显像,评估特定染料的摄取和保留。冷冻组织切片证实染料在组织和细胞水平上的积累。检测外周血中加入宫颈癌细胞的循环肿瘤细胞。结果:培养的宫颈癌细胞、人宫颈癌细胞加标全血、人宫颈癌异种移植物和新鲜人宫颈癌组织均能特异性摄取IR-783染料,而正常细胞组织则不能。结论:IR-783在临床标本和循环血中检测宫颈癌细胞的潜力,其可进一步作为宫颈癌深部组织成像的模式剂应用于临床。

Objective:To evaluate the value of near-infrared heptanine dye IR-783 as an imaging agent for detection of human cervical cancer and the potential mechanism of IR-783 mediated imaging.Methods:This fluorescent imaging was performed on cervical cancer cells to detect uptake,accumulation,and subcellular localization of IR-783 dyes.Systemic imaging was performed on mice bearing cervical cancer xenografts and newly collected clinical cervical cancer specimens in order to assess uptake and retention of specific dyes.Frozen tissue sections were used to confirm the accumulation of dye in cancer tissues and cells.The tumor cells in patients’peripheral blood were detected after adding the cervical cancer cells.Results:IR-783 dye could be specifically uptaken by cultured cervical cancer cells,labeled cancer cells from whole blood,tumour xenografts and human cervical cancer tissues,but it could not be uptaken by normal tissues and cells.Conclusion:IR-783 has the potential to detect cervical cancer cells in clinical specimens and circulating blood,and it can be further used as a model agent for deep tissue imaging in cervical cancer patients.