基于苯并呋咱胺类衍生物的碱性荧光探针

The PH Fluorescent Probe Based on Benzofuroxan Amine Derivatives

以4-氯-7-硝基苯并呋咱(NBD-Cl)为原料,设计、合成了3种荧光化合物(NBD-N、NBD-NH、NBD-NH2),通过质谱、核磁共振对其结构进行了表征.研究表明:这3种化合物在pH 7.0~12.0范围内对水溶液的酸碱度具有可逆的荧光识别响应.同时,其他常见离子的共存并不影响3种化合物对pH的荧光识别.因此,这3种化合物可作为碱性pH荧光探针.

The fluorescent probes(NBD-N,NBD-NH,NBD-NH2)were designed and synthesized using 4-chloro-7-nitrobenzofurazan(NBD-Cl).The structure of the probes was characterized with mass spectrometry and nuclear magnetism.It can recognize the pH of the aqueous solution in a reversible response within the pH range of 7.0~12.0.In addition,the coexistence of other common ions does not affect the fluorescent recognition signals of the three compounds.Therefore,the three compounds can be used for alkaline pH fluorescent probes.