DEHP促进酒精结合高脂饮食诱导大鼠酒精性脂肪肝的机制

The mechanism of alcohol and high-fat diet induced alcoholic fatty liver in rats promoted by DEHP

目的研究邻苯二甲酸二(2-乙基)己酯(DEHP)促进酒精结合高脂饮食诱导的酒精性脂肪肝的作用机制。方法将60只SD大鼠随机分为6组:正常组、模型组(酒精)、酒精+DEHP 0.05 mg/kg组、酒精+DEHP 5 mg/kg组、酒精+DEHP 500 mg/kg组以及DEHP 500 mg/kg对照组。取大鼠血清及肝脏组织,用生化分析血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平,HE切片观察大鼠肝脏损伤状态,用Western blot检测肝组织中P-AMPK及lipin-1蛋白的表达水平。结果DEHP促进了血清中ALT、AST的积累,加剧了酒精结合高脂饮食诱导的小鼠病理状态,同时上调了lipin-1蛋白水平,抑制了P-AMPK蛋白水平。结论DEHP可能通过介导lipin-1和P-AMPK蛋白水平,加重大鼠酒精性脂肪肝的病理过程。

Objective To study the mechanism of di-2-ethylhexyl phthalate(DEHP)in promoting alcoholic fatty liver induced by alcohol combined with high-fat diet.Methods 60 SD male rats were divided into 6 groups randomly,normal group,model group(alcohol),alcohol+DEHP 0.05 mg/kg group,alcohol+DEHP 5 mg/kg group,alcohol+DEHP 500 mg/kg group and DEHP 500 mg/kg control group.The serum and liver tissues of rats were taken,and the levels of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in the serum were analyzed by biochemistry.The liver injury state of rats was observed by HE slices.The expression levels of P-AMPK and lipin1 protein in liver tissues were detected by Western blot.Results DEHP promoted the accumulation of ALT and AST in serum,aggravated the pathological state of mice induced by alcohol combined with high-fat diet,and at the same time increased lipin-1 protein level and inhibited P-AMPK protein level.Conclusion DEHP may aggravate the pathological process of alcoholic fatty liver rats by mediating lipin-1 and P-AMPK protein levels.