胃癌嘌呤代谢通路差异基因筛选及其与预后关系的研究

Screening of differential genes of purine metabolic pathway in gastric cancer and its relationship with prognosis

目的探讨嘌呤代谢相关基因与胃癌患者预后的关系。方法收集胃癌组织标本91例和癌旁组织标本30例,京都基因与基因组百科全书(KEGG)富集分析胃癌嘌呤代谢相关通路中的关键基因(KGS),转录组测序筛选胃癌组织与癌旁组织之间的差异表达基因(DEGs),运用一系列数据库,包括基因表达谱分析(GEPIA)和Kaplan-Meier绘图器,研究DEGs在胃癌患者中的独特表达和预后价值。结果在嘌呤代谢通路上富集85个基因,进行差异基因分析发现差异表达基因25个,上调基因15个,下调基因10个。进一步发现5个差异基因与胃癌的肿瘤分期明显相关(P<0.05)。检测到有20个基因与胃癌预后密切相关(P<0.05)。其中磷酸二酯酶8B(PDE8B)和磷酸二酯酶9A(PDE9A)对胃癌患者的生存预测显著,风险率(HR)分别为1.87和1.58。[HTH]结论[HTSS]嘌呤代谢通路上的差异基因可能是胃癌潜在的预后标志物,这为寻找胃癌生物标志物提供了新方向。

Objective To investigate the relationship between purine metabolism related genesand prognosis of patients with gastric cancer(GC).Methods Ninety-one gastric cancer tissue specimens and 30 paraneoplastic tissue specimens were collected,and the Kyoto Encyclopedia of Genes and Genomes(KEGG)was used to enrich and analyze key genes(KGS)in purine metabolism-related pathways in gastric cancer,and transcriptome sequencing was used to screen differentially expressed genes(DEGs)between gastric cancer tissues and paraneoplastic tissues,and a series of databases were used to overanalyze including Gene Expression Profiling Interactive Analysis(GEPIA)and Kaplan-Meier plotter,to investigate the unique expression and prognostic value of DEGs in gastric cancer patients.Results Eighty-five genes were enriched in the purine metabolic pathway,and differential gene analysis was performed to identify 25 differentially expressed genes,15 up-regulated genes and 10 down-regulated genes.Five differential genes were further found to be significantly associated with tumor stage in GC.Twenty genes were detected to be closely associated with gastric cancer prognosis.Among them,PDE8B and PDE9A were significantly predictive of survival in GC patients with hazard rate(HR)of 1.87 and 1.58.Conclusion Differential genes in the purine metabolic pathway may be potential prognostic markers for gastric cancer,which will provide a new direction for the search of gastric cancer biomarkers.