交泰丸组分配伍改善小鼠胰岛素瘤B细胞脂质沉积的机制研究

Mechanism of Combination of Active Compounds of Jiaotai Pill on Improving Lipid Accumulation in Insulinoma B Cells of Mice

目的:研究交泰丸中主要活性成分小檗碱(BBR)、肉桂酸(CA)及其配伍(BBR/CA)对软脂酸(PA)诱导的NIT-1胰岛B细胞内脂质沉积的影响。方法:将细胞在PA的培养基中培养24 h,建立高脂诱导的细胞内脂肪沉积模型。分为模型组、BBR组、CA组、BBR/CA组、二甲双胍(Met)组,分别给予相应药物干预,另设对照组。油红O染色法评估细胞内脂肪沉积情况;酶法检测细胞内三酰甘油(TG)含量;使用Western Blotting和实时荧光定量PCR(RT-PCR)法检测细胞腺苷酸活化蛋白激酶(AMPK)及其下游脂肪生成和脂肪酸氧化基因的表达水平,包括脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACC)、磷酸化乙酰辅酶A羧化酶(p-ACC)、肉碱酰转移酶-1(CPT-1)、固醇调节元件结合蛋白(SREBP-1c)。结果:PA诱导使NIT-1胰岛B细胞中脂肪沉积明显增加,TG含量显著升高,AMPK蛋白及其下游靶标(如p-ACC、CPT-1等)表达显著降低。同时,AMPK下游脂肪生成基因包括SREBP-1c mRNA、FAS和ACC蛋白表达显著增加。BBR单药和BBR/CA配伍处理优于CA单药,可显著逆转NIT-1胰岛B细胞中上述基因表达水平的变化。结论:在体外,交泰丸活性组分配伍可减少高脂诱导的NIT-1胰岛B细胞内脂肪沉积,其机制可能与减少脂肪合成和增加脂肪氧化有关。

Objective:To investigate the effects of berberine(BBR)and cinnamic acid(CA),the main active compounds of Jiaotai pills,and the combination of berberine and cinnamic acid(BBR/CA)on palmitic acid(PA)-induced lipid accumulation in NIT-1 pancreatic B cells.Methods:Cells were incubated in culture medium containing PA for 24 hours to establish a model of lipid accumulation induced by high fat.Then treatments with BBR,CA,the combination of BBR and CA(BBR/CA)and Metformin(Met)were performed respectively,and a control group was set up.Intracellular lipid accumulation was assessed by Oil Red O staining and TG content was measured by enzymatic assay.The expression of adenosine monophosphate-activated protein kinase(AMPK)protein and its downstream lipogenic and fatty acid oxidation genes,including fatty acid synthase(FAS),acetyl-CoA carboxylase(ACC),phosphorylation acetyl-CoA carboxylase(p-ACC),carnitine acyl transferase 1(CPT-1)and sterol regulatory element binding protein 1c(SREBP-1c)were determined by Western blot or real time polymerase chain reaction(RT-PCR).Results:PA induced an obvious lipid accumulation and a significant increase in intracellular TG content in NIT-1 pancreatic B cells.PA also induced a remarkable decrease in AMPK protein expression and its downstream targets such as p-ACC and CPT-1.Meanwhile,AMPK downstream lipogenic genes,including SREBP-1c mRNA,FAS and ACC protein expressions,were increased significantly.Treatments with BBR and BBR/CA,superior to CA,significantly reversed the above genes changes in NIT-1 pancreatic B cells.Conclusion:It can be concluded that in vitro,the active compounds of Jiaotai pills inhibits PA-induced lipid accumulation by decreasing lipogenesis and increasing lipid oxidation in NIT-1 pancreatic B cells.