油菜Ogu CMS恢复系16C外源恢复基因染色体片段SSR标记图谱构建与比较

Constructing and comparing of SSR map on introgressed segment linkaged with Rfo gene for Ogu CMS restorer line 16C

为推进Ogu CMS不育系统杂种优势利用,探知甘蓝型油菜Ogu CMS优良恢复系16C外源导入恢复基因Rfo携带的染色体片段大小并比较其与欧洲Ogu CMS恢复系R2000的异同,以Ogu CMS不育系81A与恢复系16C为双亲构建F2分离群体,结合38对依据萝卜参考基因组开发的SSR引物进行育性性状差异标记筛选,并构建Rfo基因连锁标记图谱。SSR标记筛选结果显示:12对SSR引物在F2分离群体不育与可育DNA混合池间表现出差异,其中R9SSR2416与R9SSR3326为两侧边缘标记位点;16C与R2000差异比较结果显示:11对SSR标记在16C与R2000间扩增有差异,其中R9SSR2421与R9SSR3282为16C特有标记位点,其余9对为R2000特有标记位点。综上结果表明,甘蓝型油菜Ogu CMS恢复系16C外源导入恢复基因染色体片段来源于萝卜R9染色体,片段大小约为3.30 Mb(Chromosome_R9:8480586-11779992),与欧洲Ogu CMS恢复系R2000恢复基因携带的外源染色体片段大小与来源均不同。

To push the application of Ogu CMS restorer line 16C,SSR map of introgressed segment linkaged with Rfo gene of the Ogu CMS restorer line 16C was constructed.And comparison of 16C with R2000 from Europe was carried out by SSR molecular makers.Sterile line 81A and the restorer line 16C were used as parents to develop an F2 population.38 SSR primers originated from radish reference genome were designed to screen SSR markers linked with Rfo gene in F2.Results showed 12 of the primers linking with Rfo gene.Among them,2 markers,R9SSR2416 and R9SSR3326,were at the two end sides of introgressed fragment separately.11 of the 38 primers were found to be different between 16C and R2000 by PCR.2 of the 11 markers were specific for 16C and the other 9 were specific for R2000.The introgressed segment in 16C was about 3.30 Mb(Chromosome_R9:8480586-11779992).Thus it was different from R2000 on both size and source.