甘蓝型油菜SERK基因家族的鉴定与系统进化分析

Genome-wide identification and phylogenetic analysis of SERK gene family in Brassica napus

体细胞胚胎发生类受体激酶(somatic embryogenesis receptor-like kinase,SERK)与植物体细胞胚发生,尤其是与植物非生物逆境胁迫响应有关。为鉴定甘蓝型油菜中BnaSERK基因家族成员、揭示其进化关系及其与油菜盐/旱胁迫的响应,利用生物信息学方法对甘蓝型油菜品种中双11的SERK家族成员、基因结构、进化关系、选择压力等进行系统分析,并初步分析了部分BnaSERK基因在盐/旱胁迫下的表达响应模式。结果表明:在甘蓝型油菜基因组共鉴定到24个BnaSERK基因,它们不均等地分布在15条染色体上,可分为3个亚族,具有相对保守的基因结构和保守基序,且含多种与激素和非生物胁迫响应相关的顺式作用元件。共线性分析表明,甘蓝型油菜和拟南芥、白菜、甘蓝分别有14对、44对和32对基因表现出共线性。基于比较基因组学研究,甘蓝型油菜BnaSERK基因在经历多倍体化之后,出现了不同程度的丢失现象。分析表明,BnaSERK基因家族在芸薹属物种间的进化相对保守。盐/旱胁迫下的5个BnaSERK基因在叶片中的表达模式分析结果显示,BnaA07g29610D、BnaC01g43240D、BnaCnng07810D基因在盐胁迫下表达量上调,而BnaA01g23070D、BnaA07g23390D基因则表现为下调;Bn⁃aA07g23390D、BnaA07g29610D基因在干旱胁迫下表现为上调表达,而BnaC01g43240D、BnaCnng07810D、Bn⁃aA01g23070D基因呈下调趋势。表明BnaSERK基因可能在油菜响应盐/旱胁迫的调控机制中发挥着重要作用。

Somatic embryogenesis receptor-like kinase(SERK)is related to plant somatic embryogenesis,especially plant response to abiotic stress.In order to identify members of BnaSERK gene family in Brassica napus,cultivar Zhongshuang 11 was used to study their evolutionary relationship and function on drought and salt tolerance.Expression patterns were also explored on parts of the BnaSERK genes under drought and salt stress.A total of 24 BnaSERK genes were identified in B.napus genome,which were divided into 3 sub-families and distributed unevenly on 15 chromosomes.Their conservative gene structures and motifs were found exist with various cis-acting elements related to hormones and abiotic stress.Collinearity analysis showed that respectively 14,44 and 32 genes in B.napus were collinear with Arabidopsis,B.rapa and B.oleracea.Based on comparative genomics information,BnaSERK genes in B.napus had undergone polyploidization and gene loss or expansion to varying degrees.Selection pressure analysis showed that the BnaSERK gene family was relatively conservative during evolution process among Brassica species.BnaSERK gene expression patterns in leaves under either salt or drought stress revealed their important roles in stress.Results showed that BnaA07g29610D,BnaC01g43240D and BnaCnng07810D genes were up-regulated,while BnaA01g23070D and BnaA07g23390D genes were down-regulated under salt.BnaA07g23390D and BnaA07g29610D genes were up-regulated,and BnaC01g43240D,BnaCnng07810D and BnaA01g23070D genes were down-regulated under drought stress.